PMID- 23257628
OWN - NLM
STAT- MEDLINE
DCOM- 20130809
LR  - 20130208
IS  - 1423-0216 (Electronic)
IS  - 1021-7401 (Linking)
VI  - 20
IP  - 2
DP  - 2013
TI  - Expressions of some neurotrophins and neurotrophic cytokines at site of spinal 
      cord injury in mice after vaccination with dendritic cells pulsed with homogenate 
      proteins.
PG  - 87-98
LID - 10.1159/000345522 [doi]
AB  - OBJECTIVE: Immune cells are key mediators of secondary damage following spinal 
      cord injury (SCI), and dendritic cell (DC)-based vaccines have received 
      considerable interest for treatment of SCI. We previously showed that vaccination 
      with DCs pulsed with homogenate proteins of the spinal cord (hpDCs) promotes 
      functional recovery from SCI in mice. However, the underlying molecular 
      mechanisms remain unclear. Here, changes of neurotrophins, cytokines and T cells 
      at the site of SCI in mice after vaccination with hpDCs were investigated and 
      correlated with recovery from SCI. METHODS: hpDCs, DCs (control) or PBS (control) 
      were injected intraperitoneally into injured mouse spinal cords. Functional 
      recovery of the spinal cord was measured weekly using the Basso Mouse Scale (BMS) 
      and confirmed by histological and immunohistochemical analysis. Brain-derived 
      neurotrophic factor (BDNF), neurotrophin-3 (NT-3), interleukin-4 (IL-4) and 
      interferon-gamma (IFN-gamma) levels in T cell culture supernatants and spinal cord 
      tissues were determined by ELISA. RESULTS: Eighty-four days after immunization, 
      the BMS score of the hpDCs group (6.92 +/- 0.20) was significantly higher than 
      those of the DCs and PBS groups (p < 0.01). Meanwhile, the injury area and number 
      of cysts in the hpDCs group decreased significantly compared with control groups. 
      BDNF, NT-3, IL-4 and IFN-gamma levels at the injured site as well as BDNF and NT-3 
      levels in the supernatant of cultured T cells from the hpDCs group were 
      significantly higher than in control groups (p < 0.05). CONCLUSION: These results 
      reveal that vaccination with hpDCs can promote SCI repair potentially by 
      upregulating BDNF, NT-3, IL-4 and IFN-gamma at the injury site.
CI  - Copyright (c) 2012 S. Karger AG, Basel.
FAU - Wang, Ke
AU  - Wang K
AD  - Department of Spine Surgery, Daping Hospital, Research Institute of Surgery, 
      Third Military Medical University, Chongqing, PR China.
FAU - Chao, Rui
AU  - Chao R
FAU - Guo, Qiao-Nan
AU  - Guo QN
FAU - Liu, Ming-Yong
AU  - Liu MY
FAU - Liang, Hua-Ping
AU  - Liang HP
FAU - Liu, Peng
AU  - Liu P
FAU - Zhao, Jian-Hua
AU  - Zhao JH
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20121219
PL  - Switzerland
TA  - Neuroimmunomodulation
JT  - Neuroimmunomodulation
JID - 9422763
RN  - 0 (Cytokines)
RN  - 0 (Nerve Growth Factors)
RN  - 0 (Proteins)
SB  - IM
MH  - Animals
MH  - Cytokines/*biosynthesis
MH  - Dendritic Cells/*transplantation
MH  - Disease Models, Animal
MH  - Enzyme-Linked Immunosorbent Assay
MH  - Fluorescent Antibody Technique
MH  - Immunohistochemistry
MH  - Mice
MH  - Mice, Inbred BALB C
MH  - Nerve Growth Factors/*biosynthesis
MH  - Proteins/immunology/metabolism
MH  - Recovery of Function
MH  - Spinal Cord/immunology/metabolism
MH  - Spinal Cord Injuries/immunology/*metabolism/therapy
MH  - Vaccination
EDAT- 2012/12/22 06:00
MHDA- 2013/08/10 06:00
CRDT- 2012/12/22 06:00
PHST- 2012/06/13 00:00 [received]
PHST- 2012/10/22 00:00 [accepted]
PHST- 2012/12/22 06:00 [entrez]
PHST- 2012/12/22 06:00 [pubmed]
PHST- 2013/08/10 06:00 [medline]
AID - 000345522 [pii]
AID - 10.1159/000345522 [doi]
PST - ppublish
SO  - Neuroimmunomodulation. 2013;20(2):87-98. doi: 10.1159/000345522. Epub 2012 Dec 
      19.