PMID- 23259291
OWN - NLM
STAT- MEDLINE
DCOM- 20130520
LR  - 20121224
IS  - 2096-7993 (Print)
IS  - 2096-7993 (Linking)
VI  - 26
IP  - 18
DP  - 2012 Sep
TI  - [Expression of mir-21 and mir-375 in laryngeal squamous cell carcinoma].
PG  - 788-92
AB  - OBJECTIVE: The aims of the present study are to determine the microRNA expression 
      signature in laryngeal squamous cell carcinoma (LSCC) and the association with 
      clinical features of this disease. METHOD: We analyzed the microRNA expression 
      profiles in LSCC and adjacent normal tissue. The chip results were further 
      verified by quantitative Reverse Transcriptase Polymerase Chain Reaction. We 
      analyzed the association between the expression of mir-21,mir-375 and the clinic 
      features. RESULT: Initial analysis by gene expression microarray revealed that 
      mir-21 was one of most up-regulated microRNAs in LSCC and mir-375 was 
      down-regulated. The expression of mir-21 and mir-375 were validated by qRT-PCR. 
      The expression of mir-21 was significantly raise in tumors (P < 0.01), while the 
      expression of mir-375 was decrease in tumors (P < 0.01). The up-regulation of 
      mir-21 in LSCC was shown to be associated with clinical pathologic stages of 
      these patients (P < 0.05). The high expression of mir-21 was associated with 
      clinical feature of smoking (P < 0.05). Characteristics such as age, sex, 
      clinical stages and alcohol were analyzed according to the expression of mir-21, 
      mir-375 but no difference was observed. CONCLUSION: mir-21 is a putative 
      oncogenic RNA in head and neck cancer. The mutation of mir-21 may be involved in 
      the oncogenesis and differentiation of LSCC. Our study may helpful clarify a 
      great potential for the use of mir-21 profiling as a prognostic marker in 
      predicting the post-surgical outcome.
FAU - Hu, An
AU  - Hu A
AD  - Department of Otolaryngology-Head and Neck Surgery, Renji Hospital, Institute of 
      Otolaryngology, Medical College of Shanghai Jiaotong University, Shanghai 200001, 
      China.
FAU - Jin, Xiaojie
AU  - Jin X
LA  - chi
PT  - English Abstract
PT  - Journal Article
PL  - China
TA  - Lin Chuang Er Bi Yan Hou Tou Jing Wai Ke Za Zhi
JT  - Lin chuang er bi yan hou tou jing wai ke za zhi = Journal of clinical 
      otorhinolaryngology, head, and neck surgery
JID - 101303164
RN  - 0 (MIRN21 microRNA, human)
RN  - 0 (MIRN375 microRNA, human)
RN  - 0 (MicroRNAs)
SB  - IM
MH  - Aged
MH  - Carcinoma, Squamous Cell/*genetics/metabolism/pathology
MH  - Gene Expression Profiling/methods
MH  - Gene Expression Regulation, Neoplastic
MH  - Humans
MH  - Laryngeal Neoplasms/*genetics/metabolism/pathology
MH  - MicroRNAs/genetics/*metabolism
MH  - Middle Aged
MH  - Neoplasm Staging
MH  - Oligonucleotide Array Sequence Analysis
EDAT- 2012/12/25 06:00
MHDA- 2013/05/22 06:00
CRDT- 2012/12/25 06:00
PHST- 2012/12/25 06:00 [entrez]
PHST- 2012/12/25 06:00 [pubmed]
PHST- 2013/05/22 06:00 [medline]
PST - ppublish
SO  - Lin Chuang Er Bi Yan Hou Tou Jing Wai Ke Za Zhi. 2012 Sep;26(18):788-92.