PMID- 23260028
OWN - NLM
STAT- MEDLINE
DCOM- 20140603
LR  - 20131031
IS  - 1873-2232 (Electronic)
IS  - 0378-4320 (Linking)
VI  - 137
IP  - 1-2
DP  - 2013 Feb
TI  - Improvement of conception rate in postpartum flaxseed supplemented buffalo with 
      Ovsynch+CIDR protocol.
PG  - 15-22
LID - S0378-4320(12)00379-X [pii]
LID - 10.1016/j.anireprosci.2012.11.012 [doi]
AB  - The present study was conducted on lactating Murrah buffalo to assess the effect 
      of crushed flaxseed (a source of omega-3 fatty acids) supplementation (300g/100kg 
      bwt/day for 60 days), over and above the routine feed, on luteolytic signal 
      (PGF2alpha), luteal function (progesterone) and conception rate. In first experiment, 
      on day 50 post-calving, six non-supplemented buffalo were treated to synchronize 
      time of ovulation using an Ovsynch+Controlled Internal Drug Release (CIDR) 
      protocol followed by intravenous oxytocin treatment (OT; 100IU) on day 15 
      post-ovulation. Blood samples were collected at 15min interval, 1h before to 4h 
      after OT challenge. Thereafter, the same buffalo were supplemented with flaxseed, 
      treated to synchronize time of ovulation starting on day 35 post-supplementation 
      using the same protocol and subjected to OT treatment and blood sampling on day 
      15 post-ovulation. The PGF2alpha response was measured as the venous concentration of 
      13,14-dihydro-15-keto PGF2alpha (PGFM). The mean hourly concentration of PGFM 
      subsequent to flaxseed supplemented was less (P<0.05) than in the 
      pre-supplementation period at all the occasions. Flaxseed supplementation did not 
      affect plasma fatty acids and other plasma metabolites except for an increase 
      (P<0.05) in plasma cholesterol and plasma alanine transaminase. In the second 
      experiment, 31 buffalo were randomly assigned to a control (n=16) and flaxseed 
      supplemented (n=15) group. The latter group was supplemented with flaxseed 
      starting from day 15 post-calving. On day 50-post-calving, buffalo of both groups 
      were treated to synchronize time of ovulation among animals as described for the 
      first experiment followed by artificial insemination (AI). Post-AI luteal phase 
      plasma progesterone was greater (P<0.05) in the supplemented group compared to 
      controls. Conception rate on day 63 post-AI was 66.7% in supplemented and 31.2% 
      in controls (P<0.05). The present study indicated the beneficial impact of 
      dietary supplementation of crushed flaxseed on conception rate through 
      attenuation of luteolytic signal and improvement in post-breeding luteal profile.
CI  - Copyright (c) 2012 Elsevier B.V. All rights reserved.
FAU - Nazir, G
AU  - Nazir G
AD  - Department of Veterinary Gynaecology and Obstetrics, Guru Angad Dev Veterinary 
      and Animal Sciences University, Ludhiana, Punjab 141004, India. Electronic 
      address: drskuast266@gmail.com.
FAU - Ghuman, S P S
AU  - Ghuman SP
FAU - Singh, J
AU  - Singh J
FAU - Honparkhe, M
AU  - Honparkhe M
FAU - Ahuja, C S
AU  - Ahuja CS
FAU - Dhaliwal, G S
AU  - Dhaliwal GS
FAU - Sangha, M K
AU  - Sangha MK
FAU - Saijpaul, S
AU  - Saijpaul S
FAU - Agarwal, S K
AU  - Agarwal SK
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20121203
PL  - Netherlands
TA  - Anim Reprod Sci
JT  - Animal reproduction science
JID - 7807205
RN  - 27376-76-7 (15-keto-13,14-dihydroprostaglandin F2alpha)
RN  - 4G7DS2Q64Y (Progesterone)
RN  - B7IN85G1HY (Dinoprost)
SB  - IM
MH  - Animals
MH  - Buffaloes/*physiology
MH  - Chi-Square Distribution
MH  - Dietary Supplements
MH  - Dinoprost/analogs & derivatives/blood/*metabolism
MH  - Estrus Synchronization/*methods
MH  - Female
MH  - Flax/*metabolism
MH  - Insemination, Artificial/veterinary
MH  - Ovulation/*physiology
MH  - Pregnancy
MH  - Progesterone/blood
MH  - Random Allocation
MH  - Seeds/*metabolism
EDAT- 2012/12/25 06:00
MHDA- 2014/06/04 06:00
CRDT- 2012/12/25 06:00
PHST- 2012/04/21 00:00 [received]
PHST- 2012/11/17 00:00 [revised]
PHST- 2012/11/20 00:00 [accepted]
PHST- 2012/12/25 06:00 [entrez]
PHST- 2012/12/25 06:00 [pubmed]
PHST- 2014/06/04 06:00 [medline]
AID - S0378-4320(12)00379-X [pii]
AID - 10.1016/j.anireprosci.2012.11.012 [doi]
PST - ppublish
SO  - Anim Reprod Sci. 2013 Feb;137(1-2):15-22. doi: 10.1016/j.anireprosci.2012.11.012. 
      Epub 2012 Dec 3.