PMID- 23261443
OWN - NLM
STAT- MEDLINE
DCOM- 20130722
LR  - 20191210
IS  - 1090-2104 (Electronic)
IS  - 0006-291X (Linking)
VI  - 430
IP  - 3
DP  - 2013 Jan 18
TI  - Membrane localization of Junin virus glycoproteins requires cholesterol and 
      cholesterol rich membranes.
PG  - 912-7
LID - S0006-291X(12)02401-1 [pii]
LID - 10.1016/j.bbrc.2012.12.053 [doi]
AB  - Arenavirus morphogenesis and budding occurs at cellular plasma membrane; however, 
      the nature of membrane assembly sites remains poorly understood. In this study we 
      examined the effect of different cholesterol-lowering agents on Junin virus 
      (JUNV) multiplication. We found that cholesterol cell depletion reduced JUNV 
      glycoproteins (GPs) membrane expression and virus budding. Analysis of membrane 
      protein insolubility in Triton X-100 suggested that JUNV GPs associate with 
      cholesterol enriched membranes. Rafts dissociation conditions as warm detergent 
      extraction and cholesterol removal by methyl-beta-cyclodextrin compound showed to 
      impair GPs cholesterol enriched membrane association. Analysis of GPs transfected 
      cells showed similar results suggesting that membrane raft association is 
      independent of other viral proteins.
CI  - Copyright (c) 2012 Elsevier Inc. All rights reserved.
FAU - Cordo, Sandra M
AU  - Cordo SM
AD  - Laboratorio de Virologia, Departamento de Quimica Biologica, IQUIBICEN, Facultad 
      de Ciencias Exactas y Naturales, UBA, Ciudad Universitaria, Pabellon II, Piso 4, 
      1428, Buenos Aires, Argentina.
FAU - Valko, Ayelen
AU  - Valko A
FAU - Martinez, Guadalupe M
AU  - Martinez GM
FAU - Candurra, Nelida A
AU  - Candurra NA
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20121219
PL  - United States
TA  - Biochem Biophys Res Commun
JT  - Biochemical and biophysical research communications
JID - 0372516
RN  - 0 (Anticholesteremic Agents)
RN  - 0 (Membrane Glycoproteins)
RN  - 0 (Viral Envelope Proteins)
RN  - 97C5T2UQ7J (Cholesterol)
SB  - IM
MH  - Animals
MH  - Anticholesteremic Agents/pharmacology
MH  - Cell Membrane/*chemistry/*virology
MH  - Chlorocebus aethiops
MH  - Cholesterol/*chemistry
MH  - Dogs
MH  - Junin virus/*physiology
MH  - Madin Darby Canine Kidney Cells
MH  - Membrane Glycoproteins/*metabolism
MH  - Vero Cells
MH  - Viral Envelope Proteins/*metabolism
MH  - Virus Replication/drug effects/*physiology
EDAT- 2012/12/25 06:00
MHDA- 2013/07/23 06:00
CRDT- 2012/12/25 06:00
PHST- 2012/12/03 00:00 [received]
PHST- 2012/12/06 00:00 [accepted]
PHST- 2012/12/25 06:00 [entrez]
PHST- 2012/12/25 06:00 [pubmed]
PHST- 2013/07/23 06:00 [medline]
AID - S0006-291X(12)02401-1 [pii]
AID - 10.1016/j.bbrc.2012.12.053 [doi]
PST - ppublish
SO  - Biochem Biophys Res Commun. 2013 Jan 18;430(3):912-7. doi: 
      10.1016/j.bbrc.2012.12.053. Epub 2012 Dec 19.