PMID- 23264008
OWN - NLM
STAT- MEDLINE
DCOM- 20131211
LR  - 20130513
IS  - 1089-4098 (Electronic)
IS  - 1073-8584 (Linking)
VI  - 19
IP  - 3
DP  - 2013 Jun
TI  - Genetically encoded calcium indicators and astrocyte calcium microdomains.
PG  - 274-91
LID - 10.1177/1073858412468794 [doi]
AB  - The discovery of intracellular Ca(2+) signals within astrocytes has changed our 
      view of how these ubiquitous cells contribute to brain function. Classically 
      thought merely to serve supportive functions, astrocytes are increasingly thought 
      to respond to, and regulate, neurons. The use of organic Ca(2+) indicator dyes 
      such as Fluo-4 and Fura-2 has proved instrumental in the study of astrocyte 
      physiology. However, progress has recently been accelerated by the use of 
      cytosolic and membrane targeted genetically encoded calcium indicators (GECIs). 
      Herein, we review these recent findings, discuss why studying astrocyte Ca(2+) 
      signals is important to understand brain function, and summarize work that led to 
      the discovery of TRPA1 channel-mediated near-membrane Ca(2+) signals in 
      astrocytes and their indirect neuromodulatory roles at inhibitory synapses in the 
      CA1 stratum radiatum region of the hippocampus. We suggest that the use of 
      membrane-targeted and cytosolic GECIs holds great promise to explore the 
      diversity of Ca(2+) signals within single astrocytes and also to study diversity 
      of function for astrocytes in different parts of the brain.
FAU - Tong, Xiaoping
AU  - Tong X
AD  - Department of Physiology, David Geffen School of Medicine, University of 
      California Los Angeles, Los Angeles, CA 90095, USA.
FAU - Shigetomi, Eiji
AU  - Shigetomi E
FAU - Looger, Loren L
AU  - Looger LL
FAU - Khakh, Baljit S
AU  - Khakh BS
LA  - eng
GR  - NS060677/NS/NINDS NIH HHS/United States
GR  - NS071292/NS/NINDS NIH HHS/United States
PT  - Journal Article
PT  - Research Support, N.I.H., Extramural
PT  - Research Support, Non-U.S. Gov't
DEP - 20121220
PL  - United States
TA  - Neuroscientist
JT  - The Neuroscientist : a review journal bringing neurobiology, neurology and 
      psychiatry
JID - 9504819
RN  - 0 (Transient Receptor Potential Channels)
RN  - SY7Q814VUP (Calcium)
SB  - IM
MH  - Animals
MH  - Astrocytes/*cytology
MH  - Calcium/*metabolism
MH  - Calcium Signaling/physiology
MH  - Membrane Microdomains/*metabolism
MH  - Transient Receptor Potential Channels/*genetics/metabolism
OTO - NOTNLM
OT  - GAT-3
OT  - GCaMP3
OT  - GCaMP5G
OT  - GECI
OT  - TRPA1
OT  - astrocyte
OT  - branch
OT  - branchlet
OT  - calcium
OT  - endfoot
OT  - leaflet
OT  - process
EDAT- 2012/12/25 06:00
MHDA- 2013/12/16 06:00
CRDT- 2012/12/25 06:00
PHST- 2012/12/25 06:00 [entrez]
PHST- 2012/12/25 06:00 [pubmed]
PHST- 2013/12/16 06:00 [medline]
AID - 1073858412468794 [pii]
AID - 10.1177/1073858412468794 [doi]
PST - ppublish
SO  - Neuroscientist. 2013 Jun;19(3):274-91. doi: 10.1177/1073858412468794. Epub 2012 
      Dec 20.