PMID- 23265803
OWN - NLM
STAT- MEDLINE
DCOM- 20130228
LR  - 20130108
IS  - 2210-7762 (Print)
VI  - 205
IP  - 12
DP  - 2012 Dec
TI  - Analysis of genomic alterations in neuroblastoma by multiplex ligation-dependent 
      probe amplification and array comparative genomic hybridization: a comparison of 
      results.
PG  - 657-64
LID - S2210-7762(12)00271-2 [pii]
LID - 10.1016/j.cancergen.2012.11.002 [doi]
AB  - In cases of neuroblastoma, recurring genetic alterations--losses of the 1p, 3p, 
      4p, and 11q and/or gains of 1q, 2p, and 17q chromosome arms--are currently used 
      to define the therapeutic strategy in therapeutic protocols for low- and 
      intermediate-risk patients. Different genome-wide analysis techniques, such as 
      array comparative genomic hybridization (aCGH) or multiplex ligation-dependent 
      probe amplification (MLPA), have been suggested for detecting chromosome 
      segmental abnormalities. In this study, we compared the results of the two 
      technologies in the analyses of the DNA of tumor samples from 91 neuroblastoma 
      patients. Similar results were obtained with the two techniques for 75 samples 
      (82%). In five cases (5.5%), the MLPA results were not interpretable. 
      Discrepancies between the aCGH and MLPA results were observed in 11 cases (12%). 
      Among the discrepancies, a 18q21.2-qter gain and 16p11.2 and 11q14.1-q14.3 losses 
      were detected only by aCGH. The MLPA results showed that the 7p, 7q, and 14q 
      chromosome arms were affected in six cases, while in two cases, 2p and 17q gains 
      were observed; these results were confirmed by neither aCGH nor fluorescence in 
      situ hybridization (FISH) analysis. Because of the higher sensitivity and 
      specificity of genome-wide information, reasonable cost, and shorter time of aCGH 
      analysis, we recommend the aCGH procedure for the analysis of genomic alterations 
      in neuroblastoma.
CI  - Copyright (c) 2012 Elsevier Inc. All rights reserved.
FAU - Combaret, Valerie
AU  - Combaret V
AD  - Centre Leon Berard, Laboratoire de Recherche Translationnelle, Lyon, France. 
      valerie.combaret@lyon.unicancer.fr
FAU - Iacono, Isabelle
AU  - Iacono I
FAU - Brejon, Stephanie
AU  - Brejon S
FAU - Schleiermacher, Gudrun
AU  - Schleiermacher G
FAU - Pierron, Gaelle
AU  - Pierron G
FAU - Couturier, Jerome
AU  - Couturier J
FAU - Bergeron, Christophe
AU  - Bergeron C
FAU - Blay, Jean-Yves
AU  - Blay JY
LA  - eng
PT  - Comparative Study
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20121221
PL  - United States
TA  - Cancer Genet
JT  - Cancer genetics
JID - 101539150
SB  - IM
MH  - *Chromosome Aberrations
MH  - Comparative Genomic Hybridization/*methods
MH  - Genome, Human/*genetics
MH  - Humans
MH  - Infant
MH  - Multiplex Polymerase Chain Reaction/*methods
MH  - Neoplasm Staging
MH  - Neuroblastoma/*genetics/*pathology
EDAT- 2012/12/26 06:00
MHDA- 2013/03/01 06:00
CRDT- 2012/12/26 06:00
PHST- 2012/03/27 00:00 [received]
PHST- 2012/10/25 00:00 [revised]
PHST- 2012/11/04 00:00 [accepted]
PHST- 2012/12/26 06:00 [entrez]
PHST- 2012/12/26 06:00 [pubmed]
PHST- 2013/03/01 06:00 [medline]
AID - S2210-7762(12)00271-2 [pii]
AID - 10.1016/j.cancergen.2012.11.002 [doi]
PST - ppublish
SO  - Cancer Genet. 2012 Dec;205(12):657-64. doi: 10.1016/j.cancergen.2012.11.002. Epub 
      2012 Dec 21.