PMID- 23267080
OWN - NLM
STAT- MEDLINE
DCOM- 20130307
LR  - 20211021
IS  - 1091-6490 (Electronic)
IS  - 0027-8424 (Print)
IS  - 0027-8424 (Linking)
VI  - 110
IP  - 2
DP  - 2013 Jan 8
TI  - Eye opening and PSD95 are required for long-term potentiation in developing 
      superior colliculus.
PG  - 707-12
LID - 10.1073/pnas.1215854110 [doi]
AB  - The only major glutamate receptor membrane-associated guanylate kinase scaffolds 
      expressed in the young superficial superior colliculus (SC) are 
      synapse-associated protein 102 (SAP102) and postsynaptic density protein 95 
      (PSD95). In this, as in all visual brain regions examined, synaptic PSD95 
      increases rapidly following simultaneous eyelid opening (EO). We show that EO and 
      PSD95 are necessary for SC NMDA receptor (NMDAR)-dependent long-term potentiation 
      (LTP) and this LTP is eliminated or reinstated by manipulating EO. PSD95 
      knockdown (KD) in vivo blocks this LTP, but not long-term depression, and reduces 
      frequencies of miniature AMPA receptor and NMDAR currents with no change in 
      presynaptic release. Furthermore, miniature NMDAR currents after PSD95 KD show an 
      activity-triggered calcineurin sensitivity that is normally only found in the 
      pre-EO period when SAP102 binds mixed GluN2A/GluN2B NMDARs. These data indicate 
      that young SC LTP arises from PSD95 unsilencing of silent synapses, that 
      unsilencing is labile in young brain, and that even though SAP102 and PSD95 can 
      bind the same NMDARs, only PSD95 enables SC synaptic maturation.
FAU - Zhao, Jian-Ping
AU  - Zhao JP
AD  - McGovern Institute for Brain Research and Department of Brain and Cognitive 
      Science, Massachusetts Institute of Technology, Cambridge, MA 02139, USA. 
      jpzhao@mit.edu
FAU - Murata, Yasunobu
AU  - Murata Y
FAU - Constantine-Paton, Martha
AU  - Constantine-Paton M
LA  - eng
GR  - R01 EY014074/EY/NEI NIH HHS/United States
GR  - EY014074/EY/NEI NIH HHS/United States
PT  - Journal Article
PT  - Research Support, N.I.H., Extramural
DEP - 20121224
PL  - United States
TA  - Proc Natl Acad Sci U S A
JT  - Proceedings of the National Academy of Sciences of the United States of America
JID - 7505876
RN  - 0 (DNA Primers)
RN  - 0 (Disks Large Homolog 4 Protein)
RN  - 0 (Dlg3 protein, rat)
RN  - 0 (Dlg4 protein, rat)
RN  - 0 (Intracellular Signaling Peptides and Proteins)
RN  - 0 (Membrane Proteins)
RN  - 0 (Neuropeptides)
RN  - 0 (Receptors, N-Methyl-D-Aspartate)
RN  - EC 3.1.3.16 (Calcineurin)
SB  - IM
MH  - Age Factors
MH  - Animals
MH  - Blotting, Western
MH  - Calcineurin/metabolism
MH  - DNA Primers/genetics
MH  - Disks Large Homolog 4 Protein
MH  - Excitatory Postsynaptic Potentials
MH  - Eyelids/*physiology
MH  - Genetic Vectors/genetics
MH  - HEK293 Cells
MH  - Humans
MH  - Intracellular Signaling Peptides and Proteins/*metabolism
MH  - Lentivirus
MH  - Long-Term Potentiation/*physiology
MH  - Membrane Proteins/*metabolism
MH  - Mice
MH  - Neuropeptides/metabolism
MH  - Rats
MH  - Rats, Sprague-Dawley
MH  - Receptors, N-Methyl-D-Aspartate/*metabolism
MH  - Superior Colliculi/*physiology
MH  - Synapses/metabolism
PMC - PMC3545770
COIS- The authors declare no conflict of interest.
EDAT- 2012/12/26 06:00
MHDA- 2013/03/08 06:00
PMCR- 2012/12/24
CRDT- 2012/12/26 06:00
PHST- 2012/12/26 06:00 [entrez]
PHST- 2012/12/26 06:00 [pubmed]
PHST- 2013/03/08 06:00 [medline]
PHST- 2012/12/24 00:00 [pmc-release]
AID - 1215854110 [pii]
AID - 201215854 [pii]
AID - 10.1073/pnas.1215854110 [doi]
PST - ppublish
SO  - Proc Natl Acad Sci U S A. 2013 Jan 8;110(2):707-12. doi: 10.1073/pnas.1215854110. 
      Epub 2012 Dec 24.