PMID- 23268137
OWN - NLM
STAT- MEDLINE
DCOM- 20130930
LR  - 20130429
IS  - 1878-5492 (Electronic)
IS  - 0966-3274 (Linking)
VI  - 28
IP  - 2-3
DP  - 2013 Mar
TI  - Frequent hepatocyte chimerism in long-term human liver allografts independent of 
      graft outcome.
PG  - 100-5
LID - S0966-3274(12)00134-7 [pii]
LID - 10.1016/j.trim.2012.12.002 [doi]
AB  - Microchimerism after liver transplantation is considered to promote graft 
      tolerance or tissue repair, but its significance is controversial. By using 
      multiplex polymerase chain reaction (PCR) of short tandem repeat (STR) loci after 
      laser capture microdissection of hepatocyte nuclei, we compared the proportions 
      of recipient-derived hepatocytes in long-term stable liver allografts and late 
      dysfunctional allografts caused by chronic rejection or idiopathic 
      post-transplantation hepatitis. Through fluorescence in situ hybridization 
      (FISH), we also analyzed the presence of recipient-derived Y-positive hepatocytes 
      in the biopsies of livers transplanted from female donors to male recipients. The 
      study population comprised 24 pediatric liver transplant recipients who survived 
      with the initial graft, whose 10-year protocol biopsy records were available, and 
      who had normal liver function (stable graft, SG; n=13) or a late dysfunctional 
      graft (LDG; n=11) with similar follow-up periods (mean 10.8years in the SG group 
      and 11.2years in the LDG group). STR analysis revealed that hepatocyte chimerism 
      occurred in 7 of 13 (54%) SGs and 5 of 11 (45%) LDGs (p=0.68). The proportion of 
      hepatocyte chimerism was low, with a mean of 3% seen in 2 of 3 female-to-male 
      transplanted livers (one each of SG and LDG). In conclusion, hepatocyte chimerism 
      was a constant event. The extent of engraftment of recipient-derived hepatocytes 
      does not seem to correlate with the degree of hepatic injury in long-term liver 
      allografts.
CI  - Copyright (c) 2013 Elsevier B.V. All rights reserved.
FAU - Aini, Wulamujiang
AU  - Aini W
AD  - Department of Diagnostic Pathology, Kyoto University Hospital, Kyoto, Japan.
FAU - Miyagawa-Hayashino, Aya
AU  - Miyagawa-Hayashino A
FAU - Ozeki, Munetaka
AU  - Ozeki M
FAU - Tsuruyama, Tatsuaki
AU  - Tsuruyama T
FAU - Tamaki, Keiji
AU  - Tamaki K
FAU - Uemoto, Shinji
AU  - Uemoto S
FAU - Haga, Hironori
AU  - Haga H
LA  - eng
PT  - Journal Article
DEP - 20121222
PL  - Netherlands
TA  - Transpl Immunol
JT  - Transplant immunology
JID - 9309923
SB  - IM
MH  - *Chimerism
MH  - Female
MH  - Follow-Up Studies
MH  - Graft Rejection/*immunology
MH  - Hepatocytes/*immunology
MH  - Humans
MH  - Liver Transplantation/*immunology
MH  - Male
MH  - Transplantation, Homologous
MH  - Treatment Outcome
EDAT- 2012/12/27 06:00
MHDA- 2013/10/01 06:00
CRDT- 2012/12/27 06:00
PHST- 2012/10/04 00:00 [received]
PHST- 2012/11/24 00:00 [revised]
PHST- 2012/12/08 00:00 [accepted]
PHST- 2012/12/27 06:00 [entrez]
PHST- 2012/12/27 06:00 [pubmed]
PHST- 2013/10/01 06:00 [medline]
AID - S0966-3274(12)00134-7 [pii]
AID - 10.1016/j.trim.2012.12.002 [doi]
PST - ppublish
SO  - Transpl Immunol. 2013 Mar;28(2-3):100-5. doi: 10.1016/j.trim.2012.12.002. Epub 
      2012 Dec 22.