PMID- 23268347
OWN - NLM
STAT- MEDLINE
DCOM- 20130325
LR  - 20131121
IS  - 1879-0038 (Electronic)
IS  - 0378-1119 (Linking)
VI  - 516
IP  - 1
DP  - 2013 Mar 1
TI  - Cloning and characterization of hypoxia-inducible factor-1 subunits from Ascaris 
      suum - a parasitic nematode highly adapted to changes of oxygen conditions during 
      its life cycle.
PG  - 39-47
LID - S0378-1119(12)01536-3 [pii]
LID - 10.1016/j.gene.2012.12.025 [doi]
AB  - The parasitic nematode Ascaris suum successfully adapts to a significant decrease 
      in oxygen availability during its life cycle by altering its metabolic system 
      dramatically. However, little is known about the regulatory mechanisms of 
      adaptation to hypoxic environments in A. suum. In multicellular organisms, 
      hypoxia-inducible factor-1 (HIF-1), a heterodimeric transcription factor composed 
      of HIF-1alpha and HIF-1beta subunits, is a master regulator of genes involved in 
      adaptation to hypoxia. In the present study, cDNAs encoding HIF-1alpha and HIF-1beta 
      were cloned from A. suum and characterized. The full-length A. suum hif-1alpha and 
      hif-1beta cDNAs contain open reading frames encoding proteins with 832 and 436 amino 
      acids, respectively. In the deduced amino acid sequences of A. suum HIF-1alpha and 
      HIF-1beta, functional domains essential for DNA-binding, dimerization, and 
      oxygen-dependent prolyl hydroxylation were conserved. The interaction between A. 
      suum HIF-1alpha and HIF-1beta was confirmed by the yeast two-hybrid assay. Both A. suum 
      hif-1alpha and hif-1beta mRNAs were expressed at all stages examined (fertilized eggs, 
      third-stage larvae, lung-stage larvae, young adult worms, and adult muscle 
      tissue), and most abundantly in the aerobic free-living third-stage larvae, 
      followed by a gradual decrease after infection of the host. hif-1 mRNA 
      transcription was not sensitive to the oxygen environment in either third-stage 
      larvae or adult worms (muscle tissue), and was regulated in a stage-specific 
      manner. High expression of hif-1 mRNAs in third-stage larvae suggests its 
      contribution to pre-adaptation to a hypoxic environment after infection of their 
      host. Sequence analysis of 5'-upstream regions of mitochondrial complex II 
      (succinate-ubiquinone reductase/quinol-fumarate reductase) genes, which show 
      stage-specific expression and play an important role in oxygen adaptation during 
      the life cycle, revealed that all subunits except for the adult-type flavoprotein 
      subunit (Fp) possess putative hypoxia-responsive elements (HREs), suggesting that 
      they are hif-1 target genes.
CI  - Copyright (c) 2012 Elsevier B.V. All rights reserved.
FAU - Goto, Miho
AU  - Goto M
AD  - Department of Biomedical Chemistry, Graduate School of Medicine, The University 
      of Tokyo, 7-3-1 Hongo, Bunkyo-ku, Tokyo 113-0033, Japan.
FAU - Amino, Hisako
AU  - Amino H
FAU - Nakajima, Mikage
AU  - Nakajima M
FAU - Tsuji, Naotoshi
AU  - Tsuji N
FAU - Sakamoto, Kimitoshi
AU  - Sakamoto K
FAU - Kita, Kiyoshi
AU  - Kita K
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20121223
PL  - Netherlands
TA  - Gene
JT  - Gene
JID - 7706761
RN  - 0 (DNA, Complementary)
RN  - 0 (DNA, Helminth)
RN  - 0 (Flavoproteins)
RN  - 0 (Hypoxia-Inducible Factor 1, alpha Subunit)
RN  - 0 (RNA, Messenger)
RN  - 0 (Transcription Factors)
RN  - 138391-32-9 (Aryl Hydrocarbon Receptor Nuclear Translocator)
RN  - EC 1.- (Oxidoreductases)
RN  - EC 1.3.5.1 (Electron Transport Complex II)
RN  - EC 1.3.99.- (quinol fumarate reductase)
RN  - S88TT14065 (Oxygen)
SB  - IM
MH  - Amino Acid Sequence
MH  - Animals
MH  - Aryl Hydrocarbon Receptor Nuclear Translocator/*genetics/metabolism
MH  - Ascaris suum/genetics/*growth & development
MH  - Cloning, Molecular
MH  - DNA, Complementary
MH  - DNA, Helminth/genetics
MH  - Electron Transport Complex II/genetics/metabolism
MH  - Female
MH  - Flavoproteins/genetics/metabolism
MH  - Gene Expression Regulation
MH  - Hypoxia-Inducible Factor 1, alpha Subunit/*genetics/metabolism
MH  - *Life Cycle Stages
MH  - Molecular Sequence Data
MH  - Oxidoreductases/genetics/metabolism
MH  - Oxygen/*metabolism
MH  - RNA, Messenger/genetics/metabolism
MH  - Real-Time Polymerase Chain Reaction
MH  - Sequence Analysis, DNA
MH  - Transcription Factors/genetics/metabolism
MH  - Two-Hybrid System Techniques
EDAT- 2012/12/27 06:00
MHDA- 2013/03/26 06:00
CRDT- 2012/12/27 06:00
PHST- 2012/06/15 00:00 [received]
PHST- 2012/11/30 00:00 [revised]
PHST- 2012/12/03 00:00 [accepted]
PHST- 2012/12/27 06:00 [entrez]
PHST- 2012/12/27 06:00 [pubmed]
PHST- 2013/03/26 06:00 [medline]
AID - S0378-1119(12)01536-3 [pii]
AID - 10.1016/j.gene.2012.12.025 [doi]
PST - ppublish
SO  - Gene. 2013 Mar 1;516(1):39-47. doi: 10.1016/j.gene.2012.12.025. Epub 2012 Dec 23.