PMID- 23268959
OWN - NLM
STAT- MEDLINE
DCOM- 20130607
LR  - 20191112
IS  - 1880-313X (Electronic)
IS  - 0388-6107 (Linking)
VI  - 33
IP  - 6
DP  - 2012 Dec
TI  - Myostatin regulates proliferation and extracellular matrix mRNA expression in 
      NIH3T3 fibroblasts.
PG  - 355-61
AB  - The aim of this study was to clarify the effects of myostatin, which is a 
      negative regulator of skeletal muscle mass, on the proliferation of NIH3T3 
      fibroblasts and the synthesis of extracellular matrix (ECM) by them. A 
      proliferation assay revealed that myostatin attenuated cell growth at any of the 
      doses used. High doses of myostatin strongly inhibited cell proliferation. 
      Moreover, myostatin receptor, activin receptor type-2B (ActRIIB), was found to be 
      distributed on cells and it was also clarified that myostatin increased the 
      expression of cyclin-dependent kinase inhibitor p21 (p21). These results 
      suggested that a high dose of myostatin inhibits fibroblast proliferation by the 
      same mechanism as that for inhibition of myoblast proliferation. We then examined 
      the effects of myostatin on the mRNA expression of ECM molecules (decorin, 
      biglycan, type I collagen, type III collagen, type IV collagen and type V 
      collagen) by real-time PCR. Real-time PCR showed that myostatin increased the 
      mRNA of decorin, biglycan and collagen (types I, IV and V) in fibroblasts. The 
      results suggest that myostatin regulates ECM synthesis in cultured fibroblasts.
FAU - Z Hosaka, Yoshinao
AU  - Z Hosaka Y
AD  - Department of Veterinary Science, Tottori University, Japan.
FAU - Ishibashi, Mika
AU  - Ishibashi M
FAU - Wakamatsu, Jun-Ichi
AU  - Wakamatsu J
FAU - Uehara, Masato
AU  - Uehara M
FAU - Nishimura, Takanori
AU  - Nishimura T
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - Japan
TA  - Biomed Res
JT  - Biomedical research (Tokyo, Japan)
JID - 8100317
RN  - 0 (Cyclin-Dependent Kinase Inhibitor p21)
RN  - 0 (Extracellular Matrix Proteins)
RN  - 0 (Myostatin)
RN  - 0 (RNA, Messenger)
RN  - EC 2.7.11.30 (Activin Receptors, Type II)
RN  - EC 2.7.11.30 (activin receptor type II-B)
SB  - IM
MH  - Activin Receptors, Type II/genetics/metabolism
MH  - Animals
MH  - Cell Proliferation
MH  - Cyclin-Dependent Kinase Inhibitor p21/genetics/metabolism
MH  - Extracellular Matrix Proteins/*genetics/metabolism
MH  - Fibroblasts/*drug effects/*metabolism
MH  - Gene Expression Regulation/drug effects
MH  - Mice
MH  - Myostatin/*pharmacology
MH  - NIH 3T3 Cells
MH  - Protein Transport
MH  - RNA, Messenger/*metabolism
EDAT- 2012/12/28 06:00
MHDA- 2013/06/08 06:00
CRDT- 2012/12/28 06:00
PHST- 2012/12/28 06:00 [entrez]
PHST- 2012/12/28 06:00 [pubmed]
PHST- 2013/06/08 06:00 [medline]
AID - DN/JST.JSTAGE/biomedres/33.355 [pii]
AID - 10.2220/biomedres.33.355 [doi]
PST - ppublish
SO  - Biomed Res. 2012 Dec;33(6):355-61. doi: 10.2220/biomedres.33.355.