PMID- 23270897
OWN - NLM
STAT- MEDLINE
DCOM- 20130219
LR  - 20201218
IS  - 1943-7722 (Electronic)
IS  - 0002-9173 (Linking)
VI  - 139
IP  - 1
DP  - 2013 Jan
TI  - Diagnostic usefulness of p16/CDKN2A FISH in distinguishing between sarcomatoid 
      mesothelioma and fibrous pleuritis.
PG  - 39-46
LID - 10.1309/AJCPT94JVWIHBKRD [doi]
AB  - The distinction between sarcomatoid mesothelioma and fibrous pleuritis is 
      difficult based on histology, especially when the amount of tumor tissue examined 
      via biopsy is small and immunohistochemical examination is inconclusive. We 
      studied the usefulness of deletion of p16 with fluorescence in situ hybridization 
      (FISH) and p16 hypermethylation with polymerase chain reaction for the diagnosis 
      and prognosis of malignant pleural mesothelioma (MPM). We analyzed 50 MPMs, 
      including 22 sarcomatoid mesothelioma cases and 10 fibrous pleuritis cases. We 
      set the cutoff value of homozygous deletion pattern as 14.4% based on FISH 
      signaling patterns using samples of fibrous pleuritis. The percentage of 
      homozygous deletion pattern was higher than 14.4% in 55.6% of the epithelioid 
      mesotheliomas (10/18) and in all of the sarcomatoid mesotheliomas (22/22). 
      Methylation of p16 was observed in 7 (20.6%) of 34 informative cases. p16 FISH 
      analysis can be a reliable test for distinguishing between sarcomatoid 
      mesothelioma and fibrous pleuritis and a prognostic factor for MPM.
FAU - Wu, Di
AU  - Wu D
AD  - Dept of Pathology, Tokyo Women's Medical University Yachiyo Medical Center, 
      Owada-Shinden, Yachiyo, Chiba, Japan.
FAU - Hiroshima, Kenzo
AU  - Hiroshima K
FAU - Matsumoto, Shinji
AU  - Matsumoto S
FAU - Nabeshima, Kazuki
AU  - Nabeshima K
FAU - Yusa, Toshikazu
AU  - Yusa T
FAU - Ozaki, Daisuke
AU  - Ozaki D
FAU - Fujino, Michio
AU  - Fujino M
FAU - Yamakawa, Hisami
AU  - Yamakawa H
FAU - Nakatani, Yukio
AU  - Nakatani Y
FAU - Tada, Yuji
AU  - Tada Y
FAU - Shimada, Hideaki
AU  - Shimada H
FAU - Tagawa, Masatoshi
AU  - Tagawa M
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - England
TA  - Am J Clin Pathol
JT  - American journal of clinical pathology
JID - 0370470
RN  - 0 (Biomarkers, Tumor)
RN  - 0 (Cyclin-Dependent Kinase Inhibitor p16)
SB  - IM
MH  - Adult
MH  - Aged
MH  - Aged, 80 and over
MH  - Biomarkers, Tumor/genetics/metabolism
MH  - Cyclin-Dependent Kinase Inhibitor p16/*genetics/metabolism
MH  - DNA Methylation
MH  - Diagnosis, Differential
MH  - Female
MH  - Fibrosis/*diagnosis/genetics/metabolism
MH  - Gene Deletion
MH  - Gene Expression Regulation, Neoplastic
MH  - Gene Silencing
MH  - Humans
MH  - In Situ Hybridization, Fluorescence
MH  - Lung Neoplasms/*diagnosis/genetics/metabolism
MH  - Male
MH  - Mesothelioma/*diagnosis/genetics/metabolism
MH  - Mesothelioma, Malignant
MH  - Middle Aged
MH  - Pleural Neoplasms/*diagnosis/genetics/metabolism
MH  - Pleurisy/*diagnosis/genetics/metabolism
MH  - Prognosis
MH  - Sarcoma/*diagnosis/genetics/metabolism
EDAT- 2012/12/29 06:00
MHDA- 2013/02/21 06:00
CRDT- 2012/12/29 06:00
PHST- 2012/12/29 06:00 [entrez]
PHST- 2012/12/29 06:00 [pubmed]
PHST- 2013/02/21 06:00 [medline]
AID - 139/1/39 [pii]
AID - 10.1309/AJCPT94JVWIHBKRD [doi]
PST - ppublish
SO  - Am J Clin Pathol. 2013 Jan;139(1):39-46. doi: 10.1309/AJCPT94JVWIHBKRD.