PMID- 23270898
OWN - NLM
STAT- MEDLINE
DCOM- 20130219
LR  - 20141120
IS  - 1943-7722 (Electronic)
IS  - 0002-9173 (Linking)
VI  - 139
IP  - 1
DP  - 2013 Jan
TI  - Is it necessary to evaluate nuclei in HER2 FISH evaluation?
PG  - 47-54
LID - 10.1309/AJCPPXLYJVFGOV8I [doi]
AB  - A new method that simplifies the evaluation of the traditional HER2 fluorescence 
      in situ hybridization (FISH) evaluation in breast cancer was proposed. HER2 
      status was evaluated in digital images (DIs) captured from 423 invasive breast 
      cancer stained sections. All centromeric/CEP17 and HER2 gene signals obtained 
      from separated stacked DIs were manually counted on the screen. The global ratios 
      were compared with the traditional FISH evaluation and the immunohistochemical 
      status. The 2 FISH scores were convergent in 96.93% of cases, showing an "almost 
      perfect" agreement with a weighted k of 0.956 (95% confidence interval, 
      0.928-0.985). The new method evaluates at least 3 times more nuclei than 
      traditional methods and also has an almost perfect agreement with the 
      immunohistochemical scores. The proposed enhanced method substantially improves 
      HER2 FISH assessment in breast cancer biopsy specimens because the evaluation of 
      HER2/CEP17 copy numbers is more representative, easier, and faster than the 
      conventional method.
FAU - Lopez, Carlos
AU  - Lopez C
AD  - Molecular Biology and Research Section, Hospital de Tortosa Verge de la Cinta, 
      IISPV, URV, Tortosa, Spain. clopezp.ebre.ics@gencat.cat
FAU - Tomas, Barbara
AU  - Tomas B
FAU - Korzynska, Anna
AU  - Korzynska A
FAU - Bosch, Ramon
AU  - Bosch R
FAU - Salvado, Maria T
AU  - Salvado MT
FAU - Llobera, Montserrat
AU  - Llobera M
FAU - Garcia-Rojo, Marcial
AU  - Garcia-Rojo M
FAU - Alvaro, Tomas
AU  - Alvaro T
FAU - Jaen, Joaquin
AU  - Jaen J
FAU - Lejeune, Marylene
AU  - Lejeune M
LA  - eng
PT  - Comparative Study
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - England
TA  - Am J Clin Pathol
JT  - American journal of clinical pathology
JID - 0370470
RN  - EC 2.7.10.1 (ERBB2 protein, human)
RN  - EC 2.7.10.1 (Receptor, ErbB-2)
SB  - IM
MH  - Biopsy
MH  - Breast Neoplasms/diagnosis/*genetics
MH  - Carcinoma, Ductal, Breast/diagnosis/*genetics
MH  - Cell Nucleus/*genetics/pathology
MH  - Centromere/genetics/pathology
MH  - Chromosomes, Human, Pair 17
MH  - Female
MH  - Humans
MH  - Image Processing, Computer-Assisted
MH  - *In Situ Hybridization, Fluorescence
MH  - Observer Variation
MH  - Receptor, ErbB-2/*genetics
MH  - Reproducibility of Results
EDAT- 2012/12/29 06:00
MHDA- 2013/02/21 06:00
CRDT- 2012/12/29 06:00
PHST- 2012/12/29 06:00 [entrez]
PHST- 2012/12/29 06:00 [pubmed]
PHST- 2013/02/21 06:00 [medline]
AID - 139/1/47 [pii]
AID - 10.1309/AJCPPXLYJVFGOV8I [doi]
PST - ppublish
SO  - Am J Clin Pathol. 2013 Jan;139(1):47-54. doi: 10.1309/AJCPPXLYJVFGOV8I.