PMID- 23273192
OWN - NLM
STAT- MEDLINE
DCOM- 20130604
LR  - 20151119
IS  - 1165-158X (Electronic)
IS  - 0145-5680 (Linking)
VI  - 58
IP  - 1
DP  - 2012 Dec 22
TI  - Application of molecular markers for genetic discrimination of Fusarium wilt 
      pathogen races affecting chickpea and pigeonpea in major regions of India.
PG  - 55-65
AB  - (foc) and Fusarium udum (Fud) collected from major pulse growing regions of 
      India. Out of 247 bands produced by 24 Randomly Amplified Polymorphic DNA (RAPD) 
      primers in Foc isolates, 210 (85%) were polymorphic. A maximum of 14 amplicons 
      were generated by primer OPF 05 whereas minimum 7 amplicons were generated by 
      primer K7. A total of 24 alleles were produced by twelve Simple Sequence Repeats 
      (SSR) primers with an average of two alleles per marker in foc isolates. The 
      maximum number of 4 alleles was obtained with primer SSR 12. SSR amplicon size 
      ranged from 100 to 400 bp. The Unweighted Pair Group Method with Arithmetic 
      average (UPGMA) cluster analysis based on RAPD and SSR profiles grouped the 
      fourteen foc isolates into four major clusters. The universal Inter Transcribed 
      Spacer (ITS) primer pair amplified 630 bp bands in all fourteen foc isolates 
      while significant length polymorphism was obtained only when analysed by 
      restriction digestion with EcoRI and MspI enzymes. The cluster analysis of 
      ITS—RFLP grouped all 14 Foc isolates into three major clusters. Twenty four 
      RAPD primers generated a total of 226 bands (ranging 0.3 to 3.0 kb) in Fusarium 
      udum with an average of 9.4 bands per primer and a total of 27 alleles were 
      produced by twelve SSR primers with an average of 2.25 alleles per marker. All 
      isolates amplified a single band ranging from 100 to 450 bp. The universal ITS 
      primer pair amplified 650 bp bands in all fourteen fud isolates while significant 
      length polymorphism was obtained only when analysed by restriction digestion with 
      EcoRI and Hind III enzymes. The cluster analysis of ITS—RFLP grouped all 14 
      Fud isolates into three major clusters. The cluster analysis using various 
      markers show the grouping of Fusarium isolates strictly according to their 
      cultural characteristics and degree of pathogenicity and not the geographical 
      origin. This information will be helpful for pathologists and plant breeders to 
      design effective resistance breeding programs in chickpea and pigeonpea taking 
      into account the diversity in wilt pathogen.
FAU - Datta, J
AU  - Datta J
AD  - Department of Life Sciences, C.S.J.M. University, Kanpur 208 024, India.
FAU - Lal, N
AU  - Lal N
LA  - eng
PT  - Journal Article
DEP - 20121222
PL  - France
TA  - Cell Mol Biol (Noisy-le-grand)
JT  - Cellular and molecular biology (Noisy-le-Grand, France)
JID - 9216789
RN  - 0 (Biomarkers)
SB  - IM
MH  - Biomarkers
MH  - Cajanus/*microbiology
MH  - Cicer/*microbiology
MH  - Fusarium/*genetics/*pathogenicity
MH  - Genetic Variation
MH  - India
MH  - Microsatellite Repeats/genetics
MH  - Polymorphism, Restriction Fragment Length
MH  - Random Amplified Polymorphic DNA Technique
EDAT- 2013/01/01 06:00
MHDA- 2013/06/05 06:00
CRDT- 2013/01/01 06:00
PHST- 2012/06/11 00:00 [received]
PHST- 2012/06/23 00:00 [accepted]
PHST- 2013/01/01 06:00 [entrez]
PHST- 2013/01/01 06:00 [pubmed]
PHST- 2013/06/05 06:00 [medline]
PST - epublish
SO  - Cell Mol Biol (Noisy-le-grand). 2012 Dec 22;58(1):55-65.