PMID- 23274702
OWN - NLM
STAT- MEDLINE
DCOM- 20130821
LR  - 20131121
IS  - 1473-558X (Electronic)
IS  - 0959-4965 (Linking)
VI  - 24
IP  - 3
DP  - 2013 Feb 13
TI  - Effects of controlled release of brain-derived neurotrophic factor from collagen 
      gel on rat neural stem cells.
PG  - 101-7
LID - 10.1097/WNR.0b013e32835c93c5 [doi]
AB  - This study aimed to determine the effects of the controlled release of 
      brain-derived neurotrophic factor (BDNF) from collagen gel on rat neural stem 
      cells (NSCs). With two groups of daily addition of BDNF and collagen gel without 
      BDNF as controls, BDNF was tested using ELISA at different time points. In the 
      BDNF-collagen gel group, BDNF was steadily released from gels for 10 days. The 
      cell viability test and the bromodeoxyuridine incorporation assay showed that the 
      BDNF-collagen gel supported the survival and proliferation of NSCs. Compared with 
      controls, the length of processes was markedly longer and the differentiation 
      percentage from NSCs into neurons was much higher in the BDNF-collagen gel group 
      (P<0.05). These findings suggest that BDNF-collagen gel can significantly reduce 
      the amount of BDNF required for the culture of NSCs and increase the 
      differentiation percentage from NSCs into neurons.
FAU - Huang, Fei
AU  - Huang F
AD  - Department of Orthopaedics, The Fourth Affiliated Hospital of Anhui Medical 
      University, Hefei, People's Republic of China.
FAU - Yin, Zongsheng
AU  - Yin Z
FAU - Wu, Dan
AU  - Wu D
FAU - Hao, Jiqing
AU  - Hao J
LA  - eng
PT  - Journal Article
PL  - England
TA  - Neuroreport
JT  - Neuroreport
JID - 9100935
RN  - 0 (Brain-Derived Neurotrophic Factor)
RN  - 0 (Delayed-Action Preparations)
RN  - 0 (Nerve Tissue Proteins)
RN  - 9007-34-5 (Collagen)
RN  - EC 3.1.4.- (2',3'-Cyclic-Nucleotide Phosphodiesterases)
RN  - G34N38R2N1 (Bromodeoxyuridine)
SB  - IM
MH  - 2',3'-Cyclic-Nucleotide Phosphodiesterases/metabolism
MH  - Analysis of Variance
MH  - Animals
MH  - Brain-Derived Neurotrophic Factor/metabolism/*pharmacology
MH  - Bromodeoxyuridine/metabolism
MH  - Cell Count
MH  - Cell Differentiation/drug effects
MH  - Cell Proliferation/drug effects
MH  - Cells, Cultured
MH  - Collagen/pharmacology
MH  - Delayed-Action Preparations
MH  - Embryo, Mammalian
MH  - Female
MH  - Gene Expression Regulation/drug effects
MH  - Kinetics
MH  - Neocortex/cytology
MH  - Nerve Tissue Proteins/metabolism
MH  - Neural Stem Cells/*drug effects
MH  - Pregnancy
MH  - Rats
MH  - Rats, Sprague-Dawley
MH  - Time Factors
EDAT- 2013/01/01 06:00
MHDA- 2013/08/22 06:00
CRDT- 2013/01/01 06:00
PHST- 2013/01/01 06:00 [entrez]
PHST- 2013/01/01 06:00 [pubmed]
PHST- 2013/08/22 06:00 [medline]
AID - 10.1097/WNR.0b013e32835c93c5 [doi]
PST - ppublish
SO  - Neuroreport. 2013 Feb 13;24(3):101-7. doi: 10.1097/WNR.0b013e32835c93c5.