PMID- 23275823
OWN - NLM
STAT- PubMed-not-MEDLINE
DCOM- 20130101
LR  - 20211021
IS  - 2008-2010 (Print)
IS  - 2008-322X (Electronic)
IS  - 2008-322X (Linking)
VI  - 7
IP  - 2
DP  - 2012 Apr
TI  - Brain Derived Neurotrophic Factor and Superior Collicular Extract Regulate the 
      Expression of the 1.6 Subfamily of Voltage-gated Potassium Channels in the 
      Developing Rat Retina in vitro.
PG  - 139-47
AB  - PURPOSE: To evaluate the role of brain derived neurotrophic factor (BDNF) and 
      superior collicular extract (SCE) on the expression of the 1.6 subfamily of 
      voltage-gated potassium channels (VG Kv 1.6 channels) in retinal ganglion cells 
      (RGCs) of rats in an in vitro model. METHODS: Neonatal retinal cultures were 
      supplemented with trophic factors of interest, namely BDNF and SCE, at 0 DIV 
      (days in vitro), 6 DIV and both 0 and 6 DIV. The expression of VG Kv 1.6 channels 
      was evaluated by immunostaining with anti Kv 1.6 and immunofluorescence was 
      measured by confocal scanning laser microscopy on 4, 6, 8, 10 and 12 DIV. The 
      immunofluorescence indirectly measured the quantity of ion channels being 
      expressed. RESULTS: RGCs were identified by their soma size. BDNF and SCE 
      enhanced RGC survival by enhancing extensive neurite outgrowth, and increased the 
      expression of VG Kv 1.6 channels; the effect of SCE was more significant than 
      BDNF. Trophic factors also enhanced the survival of RGCs by increasing the 
      expression of ion channels thereby contributing to spontaneous bursts of action 
      potentials in the early stages of RGC development. CONCLUSION: The expression of 
      delayed rectifier VG Kv 1.6 channels in RGCs may determine membrane excitability 
      and responsiveness to trophic factors, this plays a key role in the refinement of 
      developing retinal circuits.
FAU - Marita Golla, Kavita
AU  - Marita Golla K
AD  - Department of Neurophysiology, National Institute of Mental Health and Neuro 
      Sciences (NIMHANS), Bangalore, India.
FAU - Ramaswamy Raju, Trichur
AU  - Ramaswamy Raju T
FAU - Chatterji, Sumanthra
AU  - Chatterji S
LA  - eng
PT  - Journal Article
PL  - United Arab Emirates
TA  - J Ophthalmic Vis Res
JT  - Journal of ophthalmic & vision research
JID - 101497643
PMC - PMC3520470
OTO - NOTNLM
OT  - Brain Derived Neurotrophic Factor
OT  - Potassium Channels
OT  - Retinal Ganglion Cells
OT  - Superior Collicular Extract
EDAT- 2013/01/01 06:00
MHDA- 2013/01/01 06:01
PMCR- 2012/04/01
CRDT- 2013/01/01 06:00
PHST- 2011/11/22 00:00 [received]
PHST- 2012/02/13 00:00 [accepted]
PHST- 2013/01/01 06:00 [entrez]
PHST- 2013/01/01 06:00 [pubmed]
PHST- 2013/01/01 06:01 [medline]
PHST- 2012/04/01 00:00 [pmc-release]
AID - JOVR-07-02-139 [pii]
PST - ppublish
SO  - J Ophthalmic Vis Res. 2012 Apr;7(2):139-47.