PMID- 23278114
OWN - NLM
STAT- MEDLINE
DCOM- 20140422
LR  - 20211021
IS  - 1520-6882 (Electronic)
IS  - 0003-2700 (Print)
IS  - 0003-2700 (Linking)
VI  - 85
IP  - 3
DP  - 2013 Feb 5
TI  - Sub 2-mum macroporous silica particles derivatized for enhanced lectin affinity 
      enrichment of glycoproteins.
PG  - 1905-12
LID - 10.1021/ac303274w [doi]
AB  - A new, mechanically stable silica microparticle with macrosized internal pores 
      (1.6 mum particles with 100 nm pores) has been developed for chromatography. The 
      particles are characterized by an extensive network of interconnected macropores 
      with a high intraparticle void volume, as observed by transmission electron 
      microscopy (TEM). They are synthesized by an aerosol assembly technique called 
      ultrasonic spray pyrolysis (USP). The particles have a high surface area for a 
      macroporous material,  approximately 200 m(2)/g, making them suitable for large biomolecular 
      separations. To demonstrate their potential for bioseparations, they have been 
      functionalized with lectins for affinity enrichment of glycoproteins. The 
      material was derivatized with two lectins, Concanavalin A (Con A) and Aleuria 
      aurantia lectin (AAL), and binding properties were tested with standard 
      glycoproteins. The columns exhibited excellent binding capacities for 
      microaffinity enrichment: Con A was able to bind 75 mug of a standard glycoprotein 
      in a 50 x 1 mm column. Following initial tests, the lectin microcolumns were 
      utilized for enrichment of glycoproteins from 1 muL volumes of blood serum 
      samples, performed in triplicate for each lectin. The enriched serum fractions 
      were subjected to side-by-side glycomic and glycoproteomic profiling analyses 
      with mass spectrometry to show that the new particles offer excellent sensitivity 
      for microscale analyses of precious biological sample materials. The unique 
      combination of the macroporous architecture and small particle diameter suggests 
      the material may have advantages for conventional modes of chromatographic 
      separation of macromolecules in an ultra-high-pressure liquid chromatography 
      (UHPLC) format.
FAU - Mann, Benjamin F
AU  - Mann BF
AD  - Department of Chemistry, Indiana University, Bloomington, Indiana 47405, United 
      States.
FAU - Mann, Amanda K P
AU  - Mann AK
FAU - Skrabalak, Sara E
AU  - Skrabalak SE
FAU - Novotny, Milos V
AU  - Novotny MV
LA  - eng
GR  - R01-GM024349/GM/NIGMS NIH HHS/United States
GR  - R01 GM024349/GM/NIGMS NIH HHS/United States
GR  - U01 CA128535/CA/NCI NIH HHS/United States
GR  - UL1 RR 02576/RR/NCRR NIH HHS/United States
GR  - U01-CA128535/CA/NCI NIH HHS/United States
PT  - Journal Article
PT  - Research Support, N.I.H., Extramural
PT  - Research Support, Non-U.S. Gov't
PT  - Research Support, U.S. Gov't, Non-P.H.S.
DEP - 20130117
PL  - United States
TA  - Anal Chem
JT  - Analytical chemistry
JID - 0370536
RN  - 0 (Glycoproteins)
RN  - 0 (Lectins)
RN  - 7631-86-9 (Silicon Dioxide)
SB  - IM
MH  - Glycoproteins/*chemistry/metabolism
MH  - Lectins/*chemistry/metabolism
MH  - *Microspheres
MH  - *Particle Size
MH  - Porosity
MH  - Protein Binding/physiology
MH  - Silicon Dioxide/*chemistry/metabolism
PMC - PMC3586544
MID - NIHMS437310
EDAT- 2013/01/03 06:00
MHDA- 2014/04/23 06:00
PMCR- 2014/02/05
CRDT- 2013/01/03 06:00
PHST- 2013/01/03 06:00 [entrez]
PHST- 2013/01/03 06:00 [pubmed]
PHST- 2014/04/23 06:00 [medline]
PHST- 2014/02/05 00:00 [pmc-release]
AID - 10.1021/ac303274w [doi]
PST - ppublish
SO  - Anal Chem. 2013 Feb 5;85(3):1905-12. doi: 10.1021/ac303274w. Epub 2013 Jan 17.