PMID- 23278571
OWN - NLM
STAT- MEDLINE
DCOM- 20130221
LR  - 20130102
IS  - 1749-6632 (Electronic)
IS  - 0077-8923 (Linking)
VI  - 1275
DP  - 2012 Dec
TI  - Antigen-specific apheresis of autoantibodies in myasthenia gravis.
PG  - 7-12
LID - 10.1111/j.1749-6632.2012.06788.x [doi]
AB  - Myasthenia gravis (MG) is an autoimmune disorder affecting the neuromuscular 
      junction, usually caused by autoantibodies against the acetylcholine receptor 
      (AChR) or the muscle-specific kinase (MuSK). Our aim is the development of a 
      therapy based on the selective extracorporeal elimination of anti-AChR or 
      anti-MuSK antibodies. To this end, the extracellular domains of the AChR subunits 
      and MuSK have been expressed in yeast to be used as adsorbents, after 
      optimization, and to obtain large quantities of proteins with near-native 
      structure. We have characterized these proteins with respect to their use as 
      specific immunoadsorbents for MG autoantibodies, and have begun large-scale 
      experiments in order to verify the feasibility of application of the method for 
      therapy. Furthermore, we have initiated animal studies to test possible toxicity 
      and safety issues of the adsorbents or the procedure itself. The successful 
      completion of the scale-up and safety tests will allow the initiation of clinical 
      trials.
CI  - (c) 2012 New York Academy of Sciences.
FAU - Lazaridis, Konstantinos
AU  - Lazaridis K
AD  - Hellenic Pasteur Institute, Athens, Greece.
FAU - Zisimopoulou, Paraskevi
AU  - Zisimopoulou P
FAU - Lagoumintzis, George
AU  - Lagoumintzis G
FAU - Skriapa, Labrini
AU  - Skriapa L
FAU - Trakas, Nikos
AU  - Trakas N
FAU - Evangelakou, Panagiota
AU  - Evangelakou P
FAU - Kanelopoulos, Ioannis
AU  - Kanelopoulos I
FAU - Grapsa, Eirini
AU  - Grapsa E
FAU - Poulas, Kostas
AU  - Poulas K
FAU - Tzartos, Socrates
AU  - Tzartos S
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - United States
TA  - Ann N Y Acad Sci
JT  - Annals of the New York Academy of Sciences
JID - 7506858
RN  - 0 (Autoantibodies)
RN  - 0 (Autoantigens)
RN  - 0 (Immunosorbents)
RN  - 0 (Receptors, Cholinergic)
SB  - IM
MH  - Autoantibodies/immunology/*isolation & purification
MH  - Autoantigens/*immunology
MH  - *Blood Component Removal
MH  - Humans
MH  - Immunosorbents
MH  - Myasthenia Gravis/immunology/*therapy
MH  - Receptors, Cholinergic/immunology
EDAT- 2013/01/03 06:00
MHDA- 2013/02/22 06:00
CRDT- 2013/01/03 06:00
PHST- 2013/01/03 06:00 [entrez]
PHST- 2013/01/03 06:00 [pubmed]
PHST- 2013/02/22 06:00 [medline]
AID - 10.1111/j.1749-6632.2012.06788.x [doi]
PST - ppublish
SO  - Ann N Y Acad Sci. 2012 Dec;1275:7-12. doi: 10.1111/j.1749-6632.2012.06788.x.