PMID- 23279974
OWN - NLM
STAT- MEDLINE
DCOM- 20130708
LR  - 20130128
IS  - 1873-6815 (Electronic)
IS  - 0531-5565 (Linking)
VI  - 48
IP  - 2
DP  - 2013 Feb
TI  - Conservation of honey bee (Apis mellifera) sperm phospholipids during storage in 
      the bee queen--a TLC/MALDI-TOF MS study.
PG  - 213-22
LID - S0531-5565(12)00335-X [pii]
LID - 10.1016/j.exger.2012.12.009 [doi]
AB  - The honey bee (Apis mellifera) is characterized by a high degree of phenotypic 
      plasticity of senescence-related processes, and has therefore become a model 
      organism of gerontological research. Sperm of honey bee drones can remain fertile 
      for several years within the storage organ of queens. The reason for this 
      longevity is unknown, but the suppression of lipid peroxidation seems to play a 
      decisive role. Here, we examined the questions of whether spermatheca- and in 
      vitro-stored honey bee sperm are indeed resistant to lipid peroxidation, and 
      whether the nature of sperm lipids could explain this resistance. The lipid 
      composition of bee sperm was determined by matrix-assisted laser desorption and 
      ionization time-of-flight (MALDI-TOF) mass spectrometry (MS) combined with 
      thin-layer chromatography (TLC). The positive ion mass spectra of drone sperm 
      lipids are dominated by two glycerophosphocholine (GPC) species, although small 
      amounts of sphingomyelins (SM) and glycerophosphoethanolamines (GPE) are also 
      detectable after TLC. Alkyl/acyl and alkenyl/acyl compounds of GPC, and 
      alkyl/acyl as well as diacyl compounds of GPE were detected containing oleyl, 
      oleoyl, palmityl and palmitoyl as the most abundant residues. Assignments of all 
      compounds have been additionally verified by enzymatic digestion and exposition 
      to HCl. During incubation of sperm in the presence of air, characteristic lipid 
      oxidation products such as lysophosphatidylcholine (LPC) appear. Inside the 
      spermatheca, however, sperm lipids are obviously protected from oxidation and 
      their composition does not change, even if they are stored over years. Our data 
      support the view that the membrane composition of honey bee sperm could help to 
      explain the extraordinary longevity of these cells.
CI  - Copyright (c) 2012 Elsevier Inc. All rights reserved.
FAU - Wegener, Jakob
AU  - Wegener J
AD  - Institute for Bee Research, Friedrich-Engels-Strasse 32, D-16540 Hohen Neuendorf, 
      Germany.
FAU - Zschornig, Kristin
AU  - Zschornig K
FAU - Onischke, Kristin
AU  - Onischke K
FAU - Fuchs, Beate
AU  - Fuchs B
FAU - Schiller, Jurgen
AU  - Schiller J
FAU - Muller, Karin
AU  - Muller K
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20121229
PL  - England
TA  - Exp Gerontol
JT  - Experimental gerontology
JID - 0047061
RN  - 0 (Lysophosphatidylcholines)
RN  - 0 (Phosphatidylcholines)
RN  - 0 (Phosphatidylethanolamines)
RN  - 0 (Phospholipids)
RN  - 0 (Sphingomyelins)
RN  - 39382-08-6 (phosphatidylethanolamine)
SB  - IM
MH  - Animals
MH  - Bees/*metabolism
MH  - Cell Survival
MH  - *Chromatography, Thin Layer
MH  - Female
MH  - *Lipid Peroxidation
MH  - Lysophosphatidylcholines/metabolism
MH  - Male
MH  - Oxidation-Reduction
MH  - Phosphatidylcholines/metabolism
MH  - Phosphatidylethanolamines/metabolism
MH  - Phospholipids/*metabolism
MH  - *Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization
MH  - Spermatozoa/*metabolism
MH  - Sphingomyelins/metabolism
MH  - Time Factors
EDAT- 2013/01/03 06:00
MHDA- 2013/07/09 06:00
CRDT- 2013/01/03 06:00
PHST- 2012/07/14 00:00 [received]
PHST- 2012/12/18 00:00 [revised]
PHST- 2012/12/22 00:00 [accepted]
PHST- 2013/01/03 06:00 [entrez]
PHST- 2013/01/03 06:00 [pubmed]
PHST- 2013/07/09 06:00 [medline]
AID - S0531-5565(12)00335-X [pii]
AID - 10.1016/j.exger.2012.12.009 [doi]
PST - ppublish
SO  - Exp Gerontol. 2013 Feb;48(2):213-22. doi: 10.1016/j.exger.2012.12.009. Epub 2012 
      Dec 29.