PMID- 23280106
OWN - NLM
STAT- MEDLINE
DCOM- 20130605
LR  - 20231115
IS  - 1557-7988 (Electronic)
IS  - 0076-6879 (Linking)
VI  - 519
DP  - 2013
TI  - FCS in STED microscopy: studying the nanoscale of lipid membrane dynamics.
PG  - 1-38
LID - B978-0-12-405539-1.00001-4 [pii]
LID - 10.1016/B978-0-12-405539-1.00001-4 [doi]
AB  - Details of molecular membrane dynamics in living cells such as lipid-protein 
      interactions or the incorporation of molecules into lipid "rafts" are often 
      hidden to the observer because of the limited spatial resolution of conventional 
      far-field optical microscopy. Fortunately, the superior spatial resolution of 
      far-field stimulated-emission-depletion (STED) nanoscopy allows gaining new 
      insights. Applying fluorescence correlation spectroscopy (FCS) in focal spots 
      continuously tuned down to 30 nm in diameter distinguishes free from anomalous 
      molecular diffusion due to transient binding, as for the diffusion of fluorescent 
      phosphoglycero- and sphingolipid analogs in the plasma membrane of living cells. 
      STED-FCS data recorded at different environmental conditions and on different 
      lipid analogs reveal molecular details of the observed nanoscale trapping. 
      Dependencies on the molecular structure of the lipids point to the distinct 
      connectivity of the various lipids to initiate or assist cellular signaling 
      events, but also outline strong differences to the characteristics of 
      liquid-ordered and disordered phase separation in model membranes. STED-FCS is a 
      highly sensitive and exceptional tool to study the membrane organization by 
      introducing a new class of nanoscale biomolecular studies.
CI  - Copyright (c) 2013 Elsevier Inc. All rights reserved.
FAU - Mueller, Veronika
AU  - Mueller V
AD  - Department of Nanobiophotonics, Max Planck Institute for Biophysical Chemistry, 
      Gottingen, Germany.
FAU - Honigmann, Alf
AU  - Honigmann A
FAU - Ringemann, Christian
AU  - Ringemann C
FAU - Medda, Rebecca
AU  - Medda R
FAU - Schwarzmann, Gunter
AU  - Schwarzmann G
FAU - Eggeling, Christian
AU  - Eggeling C
LA  - eng
GR  - MC_UU_12010/9/MRC_/Medical Research Council/United Kingdom
GR  - MR/K01577X/1/MRC_/Medical Research Council/United Kingdom
PT  - Journal Article
PL  - United States
TA  - Methods Enzymol
JT  - Methods in enzymology
JID - 0212271
RN  - 0 (Fluorescent Dyes)
SB  - IM
MH  - Diffusion
MH  - Fluorescent Dyes
MH  - *Membrane Microdomains
MH  - Spectrometry, Fluorescence/*methods
EDAT- 2013/01/03 06:00
MHDA- 2013/06/06 06:00
CRDT- 2013/01/03 06:00
PHST- 2013/01/03 06:00 [entrez]
PHST- 2013/01/03 06:00 [pubmed]
PHST- 2013/06/06 06:00 [medline]
AID - B978-0-12-405539-1.00001-4 [pii]
AID - 10.1016/B978-0-12-405539-1.00001-4 [doi]
PST - ppublish
SO  - Methods Enzymol. 2013;519:1-38. doi: 10.1016/B978-0-12-405539-1.00001-4.