PMID- 23280567
OWN - NLM
STAT- MEDLINE
DCOM- 20130612
LR  - 20221207
IS  - 1521-4141 (Electronic)
IS  - 0014-2980 (Print)
IS  - 0014-2980 (Linking)
VI  - 43
IP  - 4
DP  - 2013 Apr
TI  - Conditional ligands for Asian HLA variants facilitate the definition of CD8+ 
      T-cell responses in acute and chronic viral diseases.
PG  - 1109-20
LID - 10.1002/eji.201243088 [doi]
AB  - Conditional ligands have enabled the high-throughput production of human 
      leukocyte antigen (HLA) libraries that present defined peptides. Immunomonitoring 
      platforms typically concentrate on restriction elements associated with European 
      ancestry, and such tools are scarce for Asian HLA variants. We report 30 novel 
      irradiation-sensitive ligands, specifically targeting South East Asian 
      populations, which provide 93, 63, and 79% coverage for HLA-A, -B, and -C, 
      respectively. Unique ligands for all 16 HLA types were constructed to provide the 
      desired soluble HLA product in sufficient yield. Peptide exchange was 
      accomplished for all variants as demonstrated by an ELISA-based MHC stability 
      assay. HLA tetramers with redirected specificity could detect antigen-specific 
      CD8(+) T-cell responses against human cytomegalovirus, hepatitis B (HBV), dengue 
      virus (DENV), and Epstein-Barr virus (EBV) infections. The potential of this 
      population-centric HLA library was demonstrated with the characterization of 
      seven novel T-cell epitopes from severe acute respiratory syndrome coronavirus, 
      HBV, and DENV. Posthoc analysis revealed that the majority of responses would be 
      more readily identified by our unbiased discovery approach than through the 
      application of state-of-the-art epitope prediction. This flow cytometry-based 
      technology therefore holds considerable promise for monitoring clinically 
      relevant antigen-specific T-cell responses in populations of distinct ethnicity.
CI  - (c) 2012 WILEY-VCH Verlag GmbH & Co. KGaA, Weinheim.
FAU - Chang, Cynthia X L
AU  - Chang CX
AD  - Department of Microbiology, National University of Singapore, Singapore.
FAU - Tan, Anthony T
AU  - Tan AT
FAU - Or, Ming Yan
AU  - Or MY
FAU - Toh, Kai Yee
AU  - Toh KY
FAU - Lim, Pei Yiing
AU  - Lim PY
FAU - Chia, Adeline S E
AU  - Chia AS
FAU - Froesig, Thomas M
AU  - Froesig TM
FAU - Nadua, Karen D
AU  - Nadua KD
FAU - Oh, Hsueh-Ling J
AU  - Oh HL
FAU - Leong, Hoe Nam
AU  - Leong HN
FAU - Hadrup, Sine R
AU  - Hadrup SR
FAU - Gehring, Adam J
AU  - Gehring AJ
FAU - Tan, Yee-Joo
AU  - Tan YJ
FAU - Bertoletti, Antonio
AU  - Bertoletti A
FAU - Grotenbreg, Gijsbert M
AU  - Grotenbreg GM
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20130214
PL  - Germany
TA  - Eur J Immunol
JT  - European journal of immunology
JID - 1273201
RN  - 0 (Epitopes, T-Lymphocyte)
RN  - 0 (HLA Antigens)
RN  - 0 (Ligands)
RN  - 0 (Peptides)
SB  - IM
MH  - Amino Acid Sequence
MH  - *Asian People
MH  - CD8-Positive T-Lymphocytes/*immunology
MH  - Epitopes, T-Lymphocyte/chemistry/*immunology
MH  - HLA Antigens/chemistry/genetics/*immunology
MH  - Humans
MH  - Ligands
MH  - Molecular Sequence Data
MH  - Peptides/chemistry/immunology
MH  - Protein Multimerization
MH  - Protein Stability
MH  - Virus Diseases/*immunology
PMC - PMC3655610
COIS- The authors declare no financial or commercial conflict of interest.
EDAT- 2013/01/03 06:00
MHDA- 2013/06/13 06:00
PMCR- 2020/04/17
CRDT- 2013/01/03 06:00
PHST- 2012/10/24 00:00 [received]
PHST- 2012/12/07 00:00 [revised]
PHST- 2012/12/21 00:00 [accepted]
PHST- 2013/01/03 06:00 [entrez]
PHST- 2013/01/03 06:00 [pubmed]
PHST- 2013/06/13 06:00 [medline]
PHST- 2020/04/17 00:00 [pmc-release]
AID - EJI2543 [pii]
AID - 10.1002/eji.201243088 [doi]
PST - ppublish
SO  - Eur J Immunol. 2013 Apr;43(4):1109-20. doi: 10.1002/eji.201243088. Epub 2013 Feb 
      14.