PMID- 23282171
OWN - NLM
STAT- MEDLINE
DCOM- 20160423
LR  - 20190402
IS  - 0304-4920 (Print)
IS  - 0304-4920 (Linking)
VI  - 55
IP  - 4
DP  - 2012 Aug 31
TI  - Deltamethrin-Induced [Ca (2)(+) ]i Rise and Death in HGB Human Glioblastoma Cells.
PG  - 294-304
LID - CJP.2012.BAB114 [pii]
LID - 10.4077/CJP.2012.BAB114 [doi]
AB  - The effect of the pesticide deltamethrin on cytosolic free Ca (2)(+) concentrations 
      ([Ca(2)(+) ]i) and viability in human glioblastoma DBTRG-05MG cells is explored. 
      [Ca(2)(+) ]i was measured in suspended cells using fura-2 as a Ca(2)(+) -sensitive 
      fluorescent dye. Deltamethrin at concentrations of 5-60 muM increased [Ca(2)(+)]i in a 
      concentration-dependent fashion. The Ca(2)(+) signal was reduced partly by removing 
      extracellular Ca (2)(+) . Deltamethrin induced Mn (2)(+) entry leading to quenching of 
      fura-2 fluorescence. Deltamethrin-induced [Ca(2)(+) ]i rise was not inhibited by 
      nifedipine, econazole, SKF96365, and phorbol 12-myristate 13 acetate, but was 
      inhibited by the protein kinase C (PKC) inhibitor GF109203X via blocking Ca(2)(+) 
      release. In Ca(2)(+) -free medium, 50 muM deltamethrin pretreatment abolished the 
      [Ca(2)(+)]i rise induced by the endoplasmic reticulum Ca(2)(+) pump inhibitor 
      thapsigargin (TG) or 2,5-di-tertbutylhydroquinone (BHQ). Conversely, pretreatment 
      with TG/BHQ abolished deltamethrin-induced [Ca(2)(+)]i rise. Inhibition of inositol 
      1,4,5-trisphosphate formation with U73122 suppressed 50% of deltamethrin-induced 
      [Ca(2)(+)]i rise. At concentrations between 10 and 80 muM deltamethrin killed cells in 
      a concentration-dependent manner. The cytotoxic effect of deltamethrin was not 
      reversed by prechelating cytosolic Ca(2)(+) with 
      1,2-bis(2-aminophenoxy)ethane-N,N,N',N'-tetraacetic acid (BAPTA). Annexin V/ 
      propidium iodide staining data suggest that deltamethrin (10-40 muM) induced 
      apoptosis in a concentrationdependent manner. Deltamethrin also increased levels 
      of reactive oxygen species. Together, in human glioblastoma cells, deltamethrin 
      induced a [Ca(2)(+)]i rise by inducing phospholipase C- and PKC-dependent Ca(2)(+) 
      release from the endoplasmic reticulum and Ca(2)(+) entry via non-store-operated Ca(2)(+) 
      channels. Deltamethrin induced cell death that might involve apoptosis via 
      mitochondrial pathways.
FAU - Hsu, Shu-Shong
AU  - Hsu SS
AD  - Department of Surgery, Kaohsiung Veterans General Hospital, Kaohsiung 81362, 
      Taiwan, Republic of China.
FAU - Chou, Chiang-Ting
AU  - Chou CT
LA  - eng
PT  - Journal Article
PL  - India
TA  - Chin J Physiol
JT  - The Chinese journal of physiology
JID - 7804502
RN  - EC 3.1.4.- (Type C Phospholipases)
RN  - SY7Q814VUP (Calcium)
SB  - IM
EIN - Chin J Physiol. 2012 Oct 31;55(5):366
MH  - Animals
MH  - Apoptosis/drug effects
MH  - *Calcium/metabolism
MH  - Cell Line, Tumor
MH  - Cell Survival/drug effects
MH  - Endoplasmic Reticulum/metabolism
MH  - *Glioblastoma
MH  - Humans
MH  - Type C Phospholipases/metabolism
EDAT- 2013/01/04 06:00
MHDA- 2016/04/24 06:00
CRDT- 2013/01/04 06:00
PHST- 2013/01/04 06:00 [entrez]
PHST- 2013/01/04 06:00 [pubmed]
PHST- 2016/04/24 06:00 [medline]
AID - CJP.2012.BAB114 [pii]
AID - 10.4077/CJP.2012.BAB114 [doi]
PST - ppublish
SO  - Chin J Physiol. 2012 Aug 31;55(4):294-304. doi: 10.4077/CJP.2012.BAB114.