PMID- 23283462
OWN - NLM
STAT- MEDLINE
DCOM- 20131119
LR  - 20190606
IS  - 1678-8060 (Electronic)
IS  - 0074-0276 (Linking)
VI  - 107 Suppl 1
DP  - 2012 Dec
TI  - Mycobacterium leprae virulence-associated peptides are indicators of exposure to 
      M. leprae in Brazil, Ethiopia and Nepal.
PG  - 112-23
LID - S0074-02762012000900018 [pii]
AB  - Silent transmission of Mycobacterium leprae, as evidenced by stable leprosy 
      incidence rates in various countries, remains a health challenge despite the 
      implementation of multidrug therapy worldwide. Therefore, the development of 
      tools for the early diagnosis of M. leprae infection should be emphasised in 
      leprosy research. As part of the continuing effort to identify antigens that have 
      diagnostic potential, unique M. leprae peptides derived from predicted 
      virulence-associated proteins (group IV.A) were identified using advanced genome 
      pattern programs and bioinformatics. Based on human leukocyte antigen 
      (HLA)-binding motifs, we selected 21 peptides that were predicted to be 
      promiscuous HLA-class I T-cell epitopes and eight peptides that were predicted to 
      be HLA-class II restricted T-cell epitopes for field-testing in Brazil, Ethiopia 
      and Nepal. High levels of interferon (IFN)-gamma were induced when peripheral blood 
      mononuclear cells (PBMCs) from tuberculoid/borderline tuberculoid leprosy 
      patients located in Brazil and Ethiopia were stimulated with the ML2055 p35 
      peptide. PBMCs that were isolated from healthy endemic controls living in areas 
      with high leprosy prevalence (EChigh) in Ethiopia also responded to the ML2055 
      p35 peptide. The Brazilian EChigh group recognised the ML1358 p20 and ML1358 p24 
      peptides. None of the peptides were recognised by PBMCs from healthy controls 
      living in non-endemic region. In Nepal, mixtures of these peptides induced the 
      production of IFN-gamma by the PBMCs of leprosy patients and EChigh. Therefore, the 
      M. leprae virulence-associated peptides identified in this study may be useful 
      for identifying exposure to M. leprae in population with differing HLA 
      polymorphisms.
FAU - Bobosha, Kidist
AU  - Bobosha K
AD  - Department of Infectious Diseases, University Medical Center, Leiden, The 
      Netherlands.
FAU - Tang, Sheila Tuyet
AU  - Tang ST
FAU - van der Ploeg-van Schip, Jolien J
AU  - van der Ploeg-van Schip JJ
FAU - Bekele, Yonas
AU  - Bekele Y
FAU - Martins, Marcia V S B
AU  - Martins MV
FAU - Lund, Ole
AU  - Lund O
FAU - Franken, Kees L M C
AU  - Franken KL
FAU - Khadge, Saraswoti
AU  - Khadge S
FAU - Pontes, Maria Araci de Andrade
AU  - Pontes MA
FAU - Goncalves, Heitor de Sa
AU  - Goncalves Hde S
FAU - Hussien, Jemal
AU  - Hussien J
FAU - Thapa, Pratibha
AU  - Thapa P
FAU - Kunwar, Chhatra B
AU  - Kunwar CB
FAU - Hagge, Deanna A
AU  - Hagge DA
FAU - Aseffa, Abraham
AU  - Aseffa A
FAU - Pessolani, Maria Cristina Vidal
AU  - Pessolani MC
FAU - Pereira, Geraldo M B
AU  - Pereira GM
FAU - Ottenhoff, Tom H M
AU  - Ottenhoff TH
FAU - Geluk, Annemieke
AU  - Geluk A
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - Brazil
TA  - Mem Inst Oswaldo Cruz
JT  - Memorias do Instituto Oswaldo Cruz
JID - 7502619
RN  - 0 (Bacterial Proteins)
RN  - 0 (Cytokines)
RN  - 0 (Epitopes, T-Lymphocyte)
RN  - 0 (Peptide Fragments)
RN  - 0 (Recombinant Proteins)
SB  - IM
MH  - Bacterial Proteins/immunology
MH  - Brazil
MH  - Computational Biology
MH  - Cytokines/*immunology
MH  - Epitope Mapping
MH  - Epitopes, T-Lymphocyte/*immunology
MH  - Ethiopia
MH  - Humans
MH  - Mycobacterium leprae/immunology/isolation & purification/*pathogenicity/virology
MH  - Nepal
MH  - Peptide Fragments/immunology
MH  - Recombinant Proteins/immunology
MH  - Virulence/*immunology
EDAT- 2013/01/11 06:00
MHDA- 2013/11/20 06:00
CRDT- 2013/01/04 06:00
PHST- 2012/03/08 00:00 [received]
PHST- 2012/07/03 00:00 [accepted]
PHST- 2013/01/04 06:00 [entrez]
PHST- 2013/01/11 06:00 [pubmed]
PHST- 2013/11/20 06:00 [medline]
AID - S0074-02762012000900018 [pii]
AID - 10.1590/s0074-02762012000900018 [doi]
PST - ppublish
SO  - Mem Inst Oswaldo Cruz. 2012 Dec;107 Suppl 1:112-23. doi: 
      10.1590/s0074-02762012000900018.