PMID- 23284908
OWN - NLM
STAT- MEDLINE
DCOM- 20130620
LR  - 20211021
IS  - 1932-6203 (Electronic)
IS  - 1932-6203 (Linking)
VI  - 7
IP  - 12
DP  - 2012
TI  - BK channels reveal novel phosphate sensitivity in SNr neurons.
PG  - e52148
LID - 10.1371/journal.pone.0052148 [doi]
LID - e52148
AB  - Whether large conductance Ca(2+)-activated potassium (BK) channels are present in 
      the substantia nigra pars reticulata (SNr) is a matter of debate. Using the 
      patch-clamp technique, we examined the functional expression of BK channels in 
      neurons of the SNr and showed that the channels were activated or inhibited by 
      internal high-energy phosphates (IHEPs) at positive and negative membrane 
      potentials, respectively. SNr neurons showed membrane potential hyperpolarization 
      under glucose-deprivation conditions which was attenuated by paxilline, a 
      specific BK channel blocker. In addition, Fluo-3 fluorescence recording detected 
      an increase in the level of internal free calcium ([Ca(2+)](i)) during ischemic 
      hyperpolarization. These results confirm that BK channels are present in SNr 
      neurons and indicate that their unique IHEP sensitivity is requisite in neuronal 
      ischemic responses. Bearing in mind that the K(ATP) channel blocker tolbutamide 
      also attenuated the hyperpolarization, we suggest that BK channels may play a 
      protective role in the basal ganglia by modulating the excitability of SNr 
      neurons along with K(ATP) channels under ischemic stresses.
FAU - Ji, Juan Juan
AU  - Ji JJ
AD  - State Key Laboratory of Brain and Cognitive Sciences, Institute of Biophysics, 
      Chinese Academy of Sciences, Beijing, China. jijj@ibp.ac.cn
FAU - Chen, Lianwan
AU  - Chen L
FAU - Duan, Xuezhi
AU  - Duan X
FAU - Song, Xueqin
AU  - Song X
FAU - Su, Wenting
AU  - Su W
FAU - Zhang, Peng
AU  - Zhang P
FAU - Li, Li
AU  - Li L
FAU - Bai, Shuyun
AU  - Bai S
FAU - Sun, Yingchun
AU  - Sun Y
FAU - Inagaki, Nobuya
AU  - Inagaki N
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20121220
PL  - United States
TA  - PLoS One
JT  - PloS one
JID - 101285081
RN  - 0 (Indoles)
RN  - 0 (Large-Conductance Calcium-Activated Potassium Channels)
RN  - 0 (Phosphates)
RN  - 3T9U9Z96L7 (paxilline)
SB  - IM
MH  - Animals
MH  - Indoles/pharmacology
MH  - Large-Conductance Calcium-Activated Potassium Channels/antagonists & 
      inhibitors/*metabolism
MH  - Membrane Potentials/drug effects
MH  - Mice
MH  - Mice, Inbred C57BL
MH  - Neurons/cytology/*metabolism
MH  - Phosphates/*metabolism
MH  - Substantia Nigra/*cytology
PMC - PMC3527394
COIS- Competing Interests: The authors have declared that no competing interests exist.
EDAT- 2013/01/04 06:00
MHDA- 2013/06/21 06:00
PMCR- 2012/12/20
CRDT- 2013/01/04 06:00
PHST- 2012/05/24 00:00 [received]
PHST- 2012/11/09 00:00 [accepted]
PHST- 2013/01/04 06:00 [entrez]
PHST- 2013/01/04 06:00 [pubmed]
PHST- 2013/06/21 06:00 [medline]
PHST- 2012/12/20 00:00 [pmc-release]
AID - PONE-D-12-14979 [pii]
AID - 10.1371/journal.pone.0052148 [doi]
PST - ppublish
SO  - PLoS One. 2012;7(12):e52148. doi: 10.1371/journal.pone.0052148. Epub 2012 Dec 20.