PMID- 23284991
OWN - NLM
STAT- MEDLINE
DCOM- 20130620
LR  - 20211021
IS  - 1932-6203 (Electronic)
IS  - 1932-6203 (Linking)
VI  - 7
IP  - 12
DP  - 2012
TI  - Chronic unpredictable stress decreases expression of brain-derived neurotrophic 
      factor (BDNF) in mouse ovaries: relationship to oocytes developmental potential.
PG  - e52331
LID - 10.1371/journal.pone.0052331 [doi]
LID - e52331
AB  - BACKGROUND: Brain-derived neurotropic factor (BDNF) was originally described in 
      the nervous system but has been shown to be expressed in ovary tissues recently, 
      acting as a paracrine/autocrine regulator required for developments of follicles 
      and oocytes. Although it is generally accepted that chronic stress impairs female 
      reproduction and decreases the expression of BDNF in limbic structures of central 
      nervous system, which contributes to mood disorder. However, it is not known 
      whether chronic stress affects oocytes developments, nor whether it affects 
      expression of BDNF in ovary. METHODS: Mice were randomly assigned into control 
      group, stressed group, BDNF-treated group and BDNF-treated stressed group. The 
      chronic unpredictable mild stress model was used to produce psychosocial stress 
      in mice, and the model was verified by open field test and 
      hypothalamic-pituitary-adrenal (HPA) axis activity. The methods of 
      immunohistochemistry and western blotting were used to detect BDNF protein level 
      and distribution. The number of retrieved oocytes, oocyte maturation, embryo 
      cleavage and the rates of blastocyst formation after parthenogenetic activation 
      were evaluated. RESULTS: Chronic unpredictable stress decreased the BDNF 
      expression in antral follicles, but didn't affect the BDNF expression in 
      primordial, primary and secondary follicles. Chronic unpredictable stress also 
      decreased the number of retrieved oocytes and the rate of blastocyst formation, 
      which was rescued by exogenous BDNF treatment. CONCLUSION: BDNF in mouse ovaries 
      may be related to the decreased number of retrieved oocytes and impaired oocytes 
      developmental potential induced by chronic unpredictable stress.
FAU - Wu, Li-Min
AU  - Wu LM
AD  - Centre for Reproductive Medicine, Anhui Provincial Hospital Affiliated to Anhui 
      Medical University, Hefei, China. wlm@ustc.edu.cn
FAU - Hu, Mei-Hong
AU  - Hu MH
FAU - Tong, Xian-Hong
AU  - Tong XH
FAU - Han, Hui
AU  - Han H
FAU - Shen, Ni
AU  - Shen N
FAU - Jin, Ren-Tao
AU  - Jin RT
FAU - Wang, Wei
AU  - Wang W
FAU - Zhou, Gui-Xiang
AU  - Zhou GX
FAU - He, Guo-Ping
AU  - He GP
FAU - Liu, Yu-Sheng
AU  - Liu YS
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20121220
PL  - United States
TA  - PLoS One
JT  - PloS one
JID - 101285081
RN  - 0 (Brain-Derived Neurotrophic Factor)
SB  - IM
MH  - Animals
MH  - Blotting, Western
MH  - Brain-Derived Neurotrophic Factor/*metabolism/*pharmacology
MH  - Female
MH  - Immunohistochemistry
MH  - Mice
MH  - Oogenesis/drug effects
MH  - Ovary/*drug effects/*metabolism
MH  - Random Allocation
MH  - Stress, Physiological/*physiology
PMC - PMC3527516
COIS- Competing Interests: The authors have declared that no competing interests exist.
EDAT- 2013/01/04 06:00
MHDA- 2013/06/21 06:00
PMCR- 2012/12/20
CRDT- 2013/01/04 06:00
PHST- 2012/08/11 00:00 [received]
PHST- 2012/11/12 00:00 [accepted]
PHST- 2013/01/04 06:00 [entrez]
PHST- 2013/01/04 06:00 [pubmed]
PHST- 2013/06/21 06:00 [medline]
PHST- 2012/12/20 00:00 [pmc-release]
AID - PONE-D-12-23942 [pii]
AID - 10.1371/journal.pone.0052331 [doi]
PST - ppublish
SO  - PLoS One. 2012;7(12):e52331. doi: 10.1371/journal.pone.0052331. Epub 2012 Dec 20.