PMID- 23285977
OWN - NLM
STAT- MEDLINE
DCOM- 20130826
LR  - 20190923
IS  - 1000-8713 (Print)
IS  - 1000-8713 (Linking)
VI  - 30
IP  - 9
DP  - 2012 Sep
TI  - [Simultaneous determination of chlorpromazine and promethazine and their main 
      metabolites by capillary electrophoresis with electrochemiluminescence].
PG  - 938-42
AB  - Based on the phenomenon that each of chlorpromazine (CPZ), promethazine (PMZ), 
      chlorpromazine sulfoxide (CPZSO) and promethazine sulfoxide (PMZSO) could enhance 
      the electrochemiluminescence (ECL) intensity of tris(2,2'-bipyridyl) ruthenium, a 
      novel and sensitive method was proposed for the simultaneous determination of 
      CPZ, PMZ and their main metabolites using capillary electrophoresis (CE) coupled 
      with ECL detection. The influences of several experimental parameters were 
      explored. The optimum experimental conditions were as follows: detection 
      potential of 1. 20 V (Ag/AgCl), 40 mmol/L of phosphate buffer solution (pH 6.5) 
      containing 5 mmol/L tris(2,2'-bipyridyl) ruthenium in ECL detection cell, running 
      buffer solution of 18 mmol/L (pH 4.8), sample injection of 8 s at 11 kV, and 
      separation voltage of 13.5 kV. The detection limits (3sigma) of this method were 
      8.3 x 10(-7) g/L for CPZ, 7.2 x 10(-6) g/L for PMZ, 1.9 x 10(-5) g/L for CPZSO 
      and 3.7 x 10(-6) g/L for PMZSO. The linear ranges of ECL intensity versus mass 
      concentration of medicaments were 7. 1 x 10(-6) - 6. 3 x 10(-3) g/L for CPZ, 7.5 
      x 10(-5) - 4.6 x 10(-3) g/L for PMZ, 9.7 x 10(-5) - 3.6 x 10(-3) g/L for CPZSO 
      and 8.1 x 10(-5) - 7.7 x 10(-3) g/L for PMZSO. The relative standard deviations 
      (RSDs) of the four target compounds were not more than 3% for ECL intensity and 
      1% for migration time. This method has the merits of simplicity, speediness, 
      sensitivity, small sample injection, and free from interference. This method was 
      successfully utilized to directly and simultaneously detect CPZ, PMZ, CPZSO and 
      PMZSO in urine samples of pet dogs.
FAU - Li, Xufei
AU  - Li X
AD  - Biochemical Engineering College, Beijing Union University, Beijing 100023, China.
FAU - Yang, Yanying
AU  - Yang Y
FAU - Zhou, Kaowen
AU  - Zhou K
LA  - chi
PT  - English Abstract
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - China
TA  - Se Pu
JT  - Se pu = Chinese journal of chromatography
JID - 9424804
RN  - 016U10PN9N (opromazine)
RN  - 7640-51-9 (promethazine sulfoxide)
RN  - FF28EJQ494 (Promethazine)
RN  - U42B7VYA4P (Chlorpromazine)
SB  - IM
MH  - Animals
MH  - Chlorpromazine/analogs & derivatives/*analysis/metabolism/urine
MH  - Dogs
MH  - Electrochemical Techniques/*methods
MH  - Electrophoresis, Capillary/*methods
MH  - *Luminescence
MH  - Promethazine/analogs & derivatives/*analysis/metabolism/urine
EDAT- 2013/01/05 06:00
MHDA- 2013/08/27 06:00
CRDT- 2013/01/05 06:00
PHST- 2013/01/05 06:00 [entrez]
PHST- 2013/01/05 06:00 [pubmed]
PHST- 2013/08/27 06:00 [medline]
AID - 10.3724/sp.j.1123.2012.04025 [doi]
PST - ppublish
SO  - Se Pu. 2012 Sep;30(9):938-42. doi: 10.3724/sp.j.1123.2012.04025.