PMID- 23287046
OWN - NLM
STAT- MEDLINE
DCOM- 20130820
LR  - 20130228
IS  - 1879-3177 (Electronic)
IS  - 0887-2333 (Linking)
VI  - 27
IP  - 2
DP  - 2013 Mar
TI  - Development of an in vitro skin sensitization test based on ROS production in 
      THP-1 cells.
PG  - 857-63
LID - S0887-2333(12)00363-3 [pii]
LID - 10.1016/j.tiv.2012.12.025 [doi]
AB  - Recently, it has been reported that reactive oxygen species (ROS) produced by 
      contact allergens can affect dendritic cell migration and contact 
      hypersensitivity. The aim of the present study was to develop a new in vitro 
      assay that could predict the skin sensitizing potential of chemicals by measuring 
      ROS production in THP-1 (human monocytic leukemia cell line) cells. THP-1 cells 
      were pre-loaded with a ROS sensitive fluorescent dye, 5-(and 6-)-chloromethyl-2', 
      7'-dichlorodihydrofluorescein diacetate, acetyl ester (CM-H2DCFDA), for 15min, 
      then incubated with test chemicals for 30min. The fluorescence intensity was 
      measured by flow cytometry. For the skin sensitizers, 25 out of 30 induced over a 
      2-fold ROS production at more than 90% of cell viability. In contrast, increases 
      were only seen in 4 out of 20 non-sensitizers. The overall accuracy for the local 
      lymph node assay (LLNA) was 82% for 50 chemicals tested. A correlation was found 
      between the estimated concentration showing 2-fold ROS production in the ROS 
      assay and the EC3 values (estimated concentration required to induce positive 
      response) of the LLNA. These results indicated that the THP-1 cell-based ROS 
      assay was a rapid and highly sensitive detection system able to predict skin 
      sensitizing potentials and potency of chemicals.
CI  - Copyright (c) 2012 Elsevier Ltd. All rights reserved.
FAU - Saito, Kazutoshi
AU  - Saito K
AD  - Safety Science Research Laboratories, Kao Corporation, 2606 Akabane, 
      Ichikai-Machi, Haga-Gun, Tochigi 321-3497, Japan. saitou.kazutoshi@kao.co.jp
FAU - Miyazawa, Masaaki
AU  - Miyazawa M
FAU - Nukada, Yuko
AU  - Nukada Y
FAU - Sakaguchi, Hitoshi
AU  - Sakaguchi H
FAU - Nishiyama, Naohiro
AU  - Nishiyama N
LA  - eng
PT  - Journal Article
DEP - 20121231
PL  - England
TA  - Toxicol In Vitro
JT  - Toxicology in vitro : an international journal published in association with 
      BIBRA
JID - 8712158
RN  - 0 (Allergens)
RN  - 0 (Haptens)
RN  - 0 (Reactive Oxygen Species)
SB  - IM
MH  - Allergens/*toxicity
MH  - Animal Testing Alternatives
MH  - Biological Assay/*methods
MH  - Cell Line
MH  - Cell Survival/drug effects
MH  - Dermatitis, Allergic Contact/*etiology
MH  - Haptens/*toxicity
MH  - Humans
MH  - Reactive Oxygen Species/*metabolism
MH  - Reproducibility of Results
EDAT- 2013/01/05 06:00
MHDA- 2013/08/21 06:00
CRDT- 2013/01/05 06:00
PHST- 2012/09/03 00:00 [received]
PHST- 2012/11/30 00:00 [revised]
PHST- 2012/12/24 00:00 [accepted]
PHST- 2013/01/05 06:00 [entrez]
PHST- 2013/01/05 06:00 [pubmed]
PHST- 2013/08/21 06:00 [medline]
AID - S0887-2333(12)00363-3 [pii]
AID - 10.1016/j.tiv.2012.12.025 [doi]
PST - ppublish
SO  - Toxicol In Vitro. 2013 Mar;27(2):857-63. doi: 10.1016/j.tiv.2012.12.025. Epub 
      2012 Dec 31.