PMID- 23289199
OWN - NLM
STAT- MEDLINE
DCOM- 20130204
LR  - 20130107
IS  - 1433-6510 (Print)
IS  - 1433-6510 (Linking)
VI  - 58
IP  - 11-12
DP  - 2012
TI  - Effect of modified single cell fixation method on cell-nuclear areas and 
      fluorescence in-situ hybridization signals.
PG  - 1277-82
AB  - BACKGROUND: Fluorescence in situ hybridization (FISH) is an irreplaceable method 
      in pre-implantation genetic diagnosis. We explored the effects of a modified 
      single cell fixation method on the cell-nuclear area and FISH signal. METHODS: 
      From January 2006 to March 2008, the blastomeres with marked nuclei from D3 
      embryos were selected. Cells were fixed with three different methods. The effects 
      of the three methods on the cell-nuclear areas and FISH signals were then 
      analyzed. RESULTS: The cell fixation rate was higher in conventional (Group B, 
      94.85%) and modified (Group C, 95.79%) Tween-20/HCl + methanol/glacial acetic 
      acid methods than in the methanol/glacial acetic acid method (Group A, 86.73%) 
      with p < 0.05. The complete signal rates in group A, B, and C were 95.3%, 93.5%, 
      and 93.4%, respectively, with p > 0.05. The mean cell-nuclear areas in groups A, 
      B, and C were 55.3, 46.2, and 49.5 microm3, respectively, with p < 0.05 in group 
      A compared with group B or C, but with p > 0.05 between Group B and C. There was 
      no significant difference in signal overlap and splitting rates between the three 
      groups. CONCLUSIONS: Modified Tween-20/HCl + methanol/glacial acetic acid method 
      fails to increase FISH signal overlap and splitting rates. It is simple and its 
      fixation time is short. It can be widely used in clinical practice.
FAU - Li, Gang
AU  - Li G
AD  - Reproductive Medical Center, First Affiliated Hospital of Zhengzhou University, 
      Zhengzhou 450052, China.
FAU - Jin, Hai-Xia
AU  - Jin HX
FAU - Xin, Zhi-Min
AU  - Xin ZM
FAU - Dai, Shan-Jun
AU  - Dai SJ
FAU - Su, Ying-Chun
AU  - Su YC
FAU - Guo, Yi-Hong
AU  - Guo YH
FAU - Sun, Ying-Pu
AU  - Sun YP
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - Germany
TA  - Clin Lab
JT  - Clinical laboratory
JID - 9705611
SB  - IM
MH  - Cell Nucleus/*ultrastructure
MH  - Humans
MH  - In Situ Hybridization, Fluorescence/*methods
MH  - *Single-Cell Analysis
EDAT- 2013/01/08 06:00
MHDA- 2013/02/05 06:00
CRDT- 2013/01/08 06:00
PHST- 2013/01/08 06:00 [entrez]
PHST- 2013/01/08 06:00 [pubmed]
PHST- 2013/02/05 06:00 [medline]
PST - ppublish
SO  - Clin Lab. 2012;58(11-12):1277-82.