PMID- 23290216
OWN - NLM
STAT- MEDLINE
DCOM- 20131216
LR  - 20130107
IS  - 0376-2491 (Print)
IS  - 0376-2491 (Linking)
VI  - 92
IP  - 40
DP  - 2012 Oct 30
TI  - [Roles of interleukin-10 differentiated dendritic cell of allergic asthma 
      patients in T-lymphocyte proliferation in vitro].
PG  - 2851-4
AB  - OBJECTIVE: To explore the roles of interleukin (IL)-10 differentiated peripheral 
      blood monocyte-derived dendritic cell (DC-10) of allergic asthma patients in 
      T-lymphocytes proliferation in vitro. METHODS: From January to June 2011, 10 
      subjects with dust mite allergic asthma treated at Third Affiliated Hospital of 
      Soochow University were enrolled. Their peripheral blood monocytes were isolated 
      by Ficoll-Hypaque solution density gradient centrifugation and adherent method. 
      And the adherent monocytes were routinely cultured with granulocyte-macrophage 
      colony-stimulating factor (GM-CSF)+interleukin-4 (IL-4)+tumor necrosis 
      factor-alpha (TNF-alpha), stimulated with/without interleukin-10 (IL-10), pulsed with 
      dust mite allergen and finally harvested. The cell surface molecules including 
      CD80, CD83, CD86, human leukocyte antigen (HLA)-DR and immunoglobulin-like 
      transcript 2 (ILT2) were detected by immunofluorescent labeling and flow 
      cytometry. And cellular functions were estimated by detecting the capacities of 
      DC uptake antigens with fluorescein isothiocyanate (FITC)-dextran capturing 
      assay. The IL-10 differentiation DC (DC-10) were cultured with autologous 
      peripheral T cells (DC-10 group), either alone (DC-TNF group) or together 
      (combined group) with autologous immunostimulatory DC (DC-TNF). And the impact of 
      this treatment on T-cell responses was assessed for each donor by 3-(4, 
      5)-dimethylthiahiazo (-z-y1)-3, 5-di-phenytetrazoliumromide (MTT) assay. The 
      production of interferon-gamma (IFN-gamma), IL-4, interleukin-5 (IL-5) and interleukin-13 
      (IL-13) were measured with the quantification of enzyme linked immunosorbent 
      assay (ELISA) kits. RESULTS: In DC-10, the levels of some mature DC's markers 
      (CD80, CD83, CD86 & HLA-DR) decreased, ILT2 increased and there were the higher 
      capacities of up-taking FITC-dextran particle (72.32%+/-2.93% vs 54.41%+/-2.95%, 
      P<0.01). Compared with the DC-TNF group (1.74+/-0.15), the T cell proliferation of 
      the DC-10 group (1.06+/-0.18) and that of the combined group (1.34+/-0.16) were 
      significantly inhibited (P<0.01). The secretion levels of IFN-gamma, IL-4, IL-5 and 
      IL-10 were (2998+/-141), (157+/-17), (2608+/-254) and (55+/-11) ng/L in the DC-10 group 
      versus (3223+/-203), (149+/-19), (2465+/-183) and (88+/-10) ng/L respectively in the 
      combined group. They were all significantly reduced versus (3639+/-209), (173+/-16), 
      (2771+/-183) and (127+/-11) ng/L in the DC-TNF group (all P<0.01). CONCLUSIONS: The 
      IL-10-treated human DC may express a tolerogenic phenotype and induce the 
      allergen tolerance of T cells. And the induction of DC-10 represents a promising 
      target for therapeutic intervention of effectively managing the clinical outcomes 
      of allergic asthma.
FAU - Tang, Jian-feng
AU  - Tang JF
AD  - Department of Respiratory Medicine, Third Affiliated Hospital, Soochow 
      University, Changzhou 213000, China.
FAU - Guan, Shu-hong
AU  - Guan SH
FAU - Wang, Zhi-gang
AU  - Wang ZG
LA  - chi
PT  - English Abstract
PT  - Journal Article
PL  - China
TA  - Zhonghua Yi Xue Za Zhi
JT  - Zhonghua yi xue za zhi
JID - 7511141
RN  - 0 (Interleukin-13)
RN  - 0 (Interleukin-5)
RN  - 130068-27-8 (Interleukin-10)
RN  - 207137-56-2 (Interleukin-4)
RN  - 82115-62-6 (Interferon-gamma)
RN  - 83869-56-1 (Granulocyte-Macrophage Colony-Stimulating Factor)
SB  - IM
MH  - Adult
MH  - Asthma/*blood
MH  - Cell Differentiation
MH  - Cell Proliferation/drug effects
MH  - Dendritic Cells/cytology/*drug effects/*metabolism
MH  - Female
MH  - Granulocyte-Macrophage Colony-Stimulating Factor/metabolism
MH  - Humans
MH  - Interferon-gamma/metabolism
MH  - Interleukin-10/*pharmacology
MH  - Interleukin-13/metabolism
MH  - Interleukin-4/metabolism
MH  - Interleukin-5/metabolism
MH  - Lymphocyte Activation/*drug effects
MH  - Male
MH  - Middle Aged
MH  - Monocytes/cytology
MH  - T-Lymphocytes/*cytology
MH  - Young Adult
EDAT- 2013/01/08 06:00
MHDA- 2013/12/18 06:00
CRDT- 2013/01/08 06:00
PHST- 2013/01/08 06:00 [entrez]
PHST- 2013/01/08 06:00 [pubmed]
PHST- 2013/12/18 06:00 [medline]
PST - ppublish
SO  - Zhonghua Yi Xue Za Zhi. 2012 Oct 30;92(40):2851-4.