PMID- 23292302
OWN - NLM
STAT- MEDLINE
DCOM- 20130829
LR  - 20191210
IS  - 1559-0720 (Electronic)
IS  - 0163-4984 (Linking)
VI  - 152
IP  - 1
DP  - 2013 Apr
TI  - Zinc deficiency induced in Swiss 3T3 cells by a low-zinc medium impairs calcium 
      entry and two mechanisms of entry are involved.
PG  - 98-104
LID - 10.1007/s12011-012-9591-6 [doi]
AB  - Zinc deficiency in 3T3 cells induced by the use of diethylenetriaminepentaacetate 
      (DTPA) has been shown to impair calcium entry associated with failure of 
      proliferation when the cells are stimulated with polypeptide growth factors (GF). 
      These functions of zinc have been evaluated here in the same clone of cells by 
      simple depletion using a low-zinc medium (0.05 mumol/L zinc) without chelator. 
      Confluent cells were maintained for 1 day in the low-zinc medium without GF, then 
      loaded with Fluo-4, and stimulated with GF. Calcium entry was measured by the 
      increase in sustained fluorescence. It was preceded by the release of stored 
      calcium as observed in the previous study using DTPA. Zinc deprivation decreased 
      calcium entry when calcium was added at 0 or 0.05 mmol/L but not when 0.1 mmol/L 
      or higher. Cell proliferation reflected similar effects of zinc and calcium 
      concentrations. In a newly acquired clone of 3T3 cells, GF did not induce 
      internal calcium release but thapsigargin (TG) did. When added in a low-calcium 
      medium, both agonists stimulated calcium entry when external calcium was added, 
      suggesting that two different mechanisms of entry were impaired by zinc 
      deficiency. Zinc deficiency produced by DTPA in the newer clones gave similar 
      results, decreasing calcium entry induced by both agonists. The effects of GF and 
      TG were not additive. The results confirm the earlier observation that zinc 
      deficiency impairs calcium entry into 3T3 cells when stimulated by GF and show 
      that the cells can take up calcium by either store-operated or receptor-operated 
      mechanisms.
FAU - O'Dell, Boyd L
AU  - O'Dell BL
AD  - Department of Biochemistry, University of Missouri, 256 Stringer Wing, Eckles 
      Hall, Columbia, MO 65211, USA. odellb@missouri.edu
FAU - Browning, Jimmy D
AU  - Browning JD
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20130105
PL  - United States
TA  - Biol Trace Elem Res
JT  - Biological trace element research
JID - 7911509
RN  - 0 (Aniline Compounds)
RN  - 0 (Chelating Agents)
RN  - 0 (Culture Media)
RN  - 0 (Enzyme Inhibitors)
RN  - 0 (Fluo 4)
RN  - 0 (Fluorescent Dyes)
RN  - 0 (Intercellular Signaling Peptides and Proteins)
RN  - 0 (Xanthenes)
RN  - 67526-95-8 (Thapsigargin)
RN  - 7A314HQM0I (Pentetic Acid)
RN  - EC 7.2.2.10 (Calcium-Transporting ATPases)
RN  - J41CSQ7QDS (Zinc)
RN  - SY7Q814VUP (Calcium)
SB  - IM
MH  - 3T3 Cells
MH  - Aniline Compounds/metabolism
MH  - Animals
MH  - Biological Transport/drug effects
MH  - Calcium/*metabolism/pharmacology
MH  - Calcium-Transporting ATPases/antagonists & inhibitors/metabolism
MH  - Cell Proliferation/drug effects
MH  - Chelating Agents/pharmacology
MH  - Culture Media/*metabolism/pharmacology
MH  - Cytosol/drug effects/*metabolism
MH  - Dose-Response Relationship, Drug
MH  - Enzyme Inhibitors/pharmacology
MH  - Fluorescent Dyes/metabolism
MH  - Intercellular Signaling Peptides and Proteins/pharmacology
MH  - Mice
MH  - Pentetic Acid/pharmacology
MH  - Thapsigargin/pharmacology
MH  - Xanthenes/metabolism
MH  - Zinc/*metabolism/pharmacology
EDAT- 2013/01/08 06:00
MHDA- 2013/08/30 06:00
CRDT- 2013/01/08 06:00
PHST- 2012/10/12 00:00 [received]
PHST- 2012/12/19 00:00 [accepted]
PHST- 2013/01/08 06:00 [entrez]
PHST- 2013/01/08 06:00 [pubmed]
PHST- 2013/08/30 06:00 [medline]
AID - 10.1007/s12011-012-9591-6 [doi]
PST - ppublish
SO  - Biol Trace Elem Res. 2013 Apr;152(1):98-104. doi: 10.1007/s12011-012-9591-6. Epub 
      2013 Jan 5.