PMID- 23292583
OWN - NLM
STAT- MEDLINE
DCOM- 20130614
LR  - 20180302
IS  - 1559-0488 (Electronic)
IS  - 1559-047X (Linking)
VI  - 34
IP  - 1
DP  - 2013 Jan-Feb
TI  - "Take" of a polymer-based autologous cultured composite "skin" on an integrated 
      temporizing dermal matrix: proof of concept.
PG  - 151-60
LID - 10.1097/BCR.0b013e31828089f9 [doi]
AB  - This study aimed to investigate the ability of an autologous cultured composite 
      skin (CCS) to close similar biodegradable temporizing matrix (BTM)-integrated 
      wounds, and its effectiveness in healing fresh full-thickness wounds after the 
      failure of cultured epithelial autograft in its two forms (sheets and 
      suspensions) to epithelialize over an integrated polymer BTM. Using a porcine 
      model, autologous split-skin grafts were harvested three of four dorsal 8 x 8 cm 
      treatment sites. These three sites were subsequently converted to full-thickness 
      wounds and BTMs were implanted. The grafts were used to produce autologous CCSs 
      for each pig. These consisted of a 1 mm thick biodegradable polymer foam scaffold 
      into which fibroblasts and keratinocytes harvested from the grafts were 
      cocultured. At Day 28, on each animal, the autologous CCSs were applied to two of 
      the integrated BTMs, an autologous split-skin graft was applied to the third 
      integrated BTM, and one CCS was applied immediately into a fresh, "naked" (no BTM 
      applied) wound. The CCSs were capable of generating a bilayer repair over the 
      naked wound's fat base and BTM-integrated wounds, which consisted of dermal 
      elements and a keratinized stratified squamous epidermis anchored with a basement 
      membrane by day 7. The CCSs behaved in different ways: either as a delivery 
      vehicle allowing similar development of a bilayer repair while the polymer foam 
      was shed from the wound, or generating a bilayer repair with the foam scaffold 
      being retained (composite "take"). These results conclude our porcine program and 
      provide proof of concept that the integrated BTM can be closed with an autologous 
      CCS. Once fully optimized, this may provide robust repair without resorting to 
      the split-skin graft, important in those cases where unburned donor site is 
      unavailable.
FAU - Dearman, Bronwyn L
AU  - Dearman BL
AD  - Skin Engineering Laboratory, Royal Adelaide Hospital, Adelaide, South Australia.
FAU - Stefani, Kristian
AU  - Stefani K
FAU - Li, Amy
AU  - Li A
FAU - Greenwood, John E
AU  - Greenwood JE
LA  - eng
PT  - Journal Article
PL  - England
TA  - J Burn Care Res
JT  - Journal of burn care & research : official publication of the American Burn 
      Association
JID - 101262774
RN  - 0 (Biocompatible Materials)
RN  - 0 (Polymers)
SB  - IM
MH  - Animals
MH  - Biocompatible Materials
MH  - Biopsy
MH  - Cells, Cultured
MH  - Dermatologic Surgical Procedures
MH  - Polymers
MH  - Skin/*injuries
MH  - Skin Transplantation/*methods
MH  - *Skin, Artificial
MH  - Swine
MH  - Transplantation, Autologous
MH  - *Wound Healing
EDAT- 2013/01/08 06:00
MHDA- 2013/06/15 06:00
CRDT- 2013/01/08 06:00
PHST- 2013/01/08 06:00 [entrez]
PHST- 2013/01/08 06:00 [pubmed]
PHST- 2013/06/15 06:00 [medline]
AID - 01253092-201301000-00020 [pii]
AID - 10.1097/BCR.0b013e31828089f9 [doi]
PST - ppublish
SO  - J Burn Care Res. 2013 Jan-Feb;34(1):151-60. doi: 10.1097/BCR.0b013e31828089f9.