PMID- 23296662
OWN - NLM
STAT- MEDLINE
DCOM- 20130613
LR  - 20190816
IS  - 1940-6029 (Electronic)
IS  - 1064-3745 (Linking)
VI  - 965
DP  - 2013
TI  - Methods for the assessment of telomere status.
PG  - 233-42
LID - 10.1007/978-1-62703-239-1_15 [doi]
AB  - Most methods for examining telomere functionality have relied on measurements of 
      telomeric DNA by hybridization or quantitative PCR. While these techniques yield 
      measures of telomeric DNA length, they generate whole-population results. 
      However, telomeric DNA lengths on different chromatids even in the same cell are 
      usually heterogeneous. Also, these measurements do not reveal whether a 
      particular telomere contains the critical minimum DNA length to be functional. 
      Therefore, in order to gain a more complete knowledge of cellular health, an 
      alternative method that reveals the functional status of each individual telomere 
      is needed. Based on the fact that a dysfunctional telomere induces a DNA damage 
      response, we developed a novel technique which combines a DNA damage marker with 
      fluorescence in situ hybridization (FISH) of telomeric DNA on metaphase 
      chromosomes to assess the functional status of individual telomeres. This 
      technique reveals not only whether the telomeric DNA in each chromatid is 
      significantly shortened, but also whether the telomere has induced a DNA damage 
      response, i.e., has become dysfunctional. We describe here in detail the 
      protocols for simultaneous assessment of telomere length and functionality.
FAU - Nakamura, Asako J
AU  - Nakamura AJ
AD  - Department of Anatomy and Cell Biology, Osaka Medical College, Takatsuki, Japan. 
      an1024@art.osaka-med.ac.jp
LA  - eng
PT  - Journal Article
PL  - United States
TA  - Methods Mol Biol
JT  - Methods in molecular biology (Clifton, N.J.)
JID - 9214969
RN  - 0 (H2AX protein, human)
RN  - 0 (Histones)
SB  - IM
MH  - Chromosomes, Human/genetics
MH  - Histones/metabolism
MH  - Humans
MH  - Image Processing, Computer-Assisted
MH  - Immunohistochemistry
MH  - In Situ Hybridization, Fluorescence/*methods
MH  - Metaphase/genetics
MH  - Telomere/*genetics
EDAT- 2013/01/09 06:00
MHDA- 2013/06/14 06:00
CRDT- 2013/01/09 06:00
PHST- 2013/01/09 06:00 [entrez]
PHST- 2013/01/09 06:00 [pubmed]
PHST- 2013/06/14 06:00 [medline]
AID - 10.1007/978-1-62703-239-1_15 [doi]
PST - ppublish
SO  - Methods Mol Biol. 2013;965:233-42. doi: 10.1007/978-1-62703-239-1_15.