PMID- 23296833
OWN - NLM
STAT- MEDLINE
DCOM- 20131025
LR  - 20130402
IS  - 1525-3163 (Electronic)
IS  - 0021-8812 (Linking)
VI  - 91
IP  - 3
DP  - 2013 Mar
TI  - Ephrin-A5 promotes bovine muscle progenitor cell migration before mitotic 
      activation.
PG  - 1086-93
LID - 10.2527/jas.2012-5728 [doi]
AB  - Satellite cells are the resident stem cell population of adult skeletal muscle 
      tissue that is responsible for growth and regeneration. The cells typically 
      congregate near the tips of the muscle fibers and in close proximity to the 
      neural muscular junction (NMJ). Ephrin-A5 is a chemotactic molecule that 
      participates in the correct positioning and formation of the NMJ. The objective 
      of the experiment was to examine the effects of ephrin-A5 signaling on bovine 
      satellite cell (BSC) biology. Primary cultures of BSC demonstrate changes in 
      velocity with time in culture that is unique to the Paired box protein 7 
      (Pax7):Myogenic factor 5 (Myf5) subpopulation. Treatment of the BSC with 
      ephrin-A5 causes a reduction (P < 0.05) in velocity with a concomitant increase 
      (P < 0.05) in directed migration. The chemoattractant properties of ephrin-A5 
      occur before myogenic differentiation 1 (MyoD) expression in the myogenic 
      precursors and are abrogated after their differentiation to committed myoblasts. 
      Ephrin-A5 induced migration appears to require components of the Ras homolog gene 
      family member A (RhoA) and Rho-associated protein kinase (ROCK) signaling 
      machinery. Supplementation of culture media with a chemical ROCK inhibitor 
      suppressed (P < 0.05) ephrin-A5 initiated BSC migration. These results contrast 
      with treatment of BSC with hepatocyte growth factor (HGF), a key modulator of 
      myogenic and motogenic activity. Treatment of BSC with HGF had no effect on cell 
      motility or migration immediately after culture establishment. Twenty-four hours 
      after culture establishment, BSC demonstrated an increase (P < 0.05) in transwell 
      migration toward HGF. These results document that temporal and spatial gradients 
      of chemokines and growth factors participate in the localization of BSC within 
      the niche.
FAU - Li, J
AU  - Li J
AD  - Department of Animal Sciences, University of Florida, Gainesville 32611, USA.
FAU - Johnson, S E
AU  - Johnson SE
LA  - eng
PT  - Journal Article
PT  - Research Support, U.S. Gov't, Non-P.H.S.
DEP - 20130107
PL  - United States
TA  - J Anim Sci
JT  - Journal of animal science
JID - 8003002
RN  - 0 (Ephrin-A5)
RN  - 67256-21-7 (Hepatocyte Growth Factor)
SB  - IM
MH  - Animals
MH  - Blotting, Western/veterinary
MH  - Cattle
MH  - *Cell Movement
MH  - Cells, Cultured
MH  - Ephrin-A5/*metabolism
MH  - Hepatocyte Growth Factor/*metabolism
MH  - Immunohistochemistry/veterinary
MH  - Male
MH  - Muscle, Skeletal/*physiology
MH  - Satellite Cells, Skeletal Muscle/*physiology
MH  - Time Factors
EDAT- 2013/01/09 06:00
MHDA- 2013/10/26 06:00
CRDT- 2013/01/09 06:00
PHST- 2013/01/09 06:00 [entrez]
PHST- 2013/01/09 06:00 [pubmed]
PHST- 2013/10/26 06:00 [medline]
AID - jas.2012-5728 [pii]
AID - 10.2527/jas.2012-5728 [doi]
PST - ppublish
SO  - J Anim Sci. 2013 Mar;91(3):1086-93. doi: 10.2527/jas.2012-5728. Epub 2013 Jan 7.