PMID- 23296886
OWN - NLM
STAT- MEDLINE
DCOM- 20130606
LR  - 20130108
IS  - 1940-6029 (Electronic)
IS  - 1064-3745 (Linking)
VI  - 968
DP  - 2013
TI  - Microscopic detection of yeasts using fluorescence in situ hybridization.
PG  - 71-82
LID - 10.1007/978-1-62703-257-5_5 [doi]
AB  - Fluorescence in situ hybridization (FISH) has been widely used for the detection 
      and identification of microorganisms in their natural environments. In this 
      chapter we describe the use of a simple FISH-based protocol to detect and 
      identify clinically relevant yeast species in culture and biological samples 
      using Cryptococcus neoformans as a model. After fixation of cells with 
      paraformaldehyde, the same are embedded in hybridization buffer containing 
      specific fluorochrome-labeled oligonucleotide probes. After incubation and a 
      subsequent washing step for removing unbound probes, samples are analyzed by 
      epifluorescence microscopy.
FAU - Inacio, Joao
AU  - Inacio J
AD  - Instituto de Higiene e Medicina Tropical, Universidade Nova de Lisboa (IHMT/UNL), 
      Lisboa, Portugal.
FAU - da Luz Martins, Maria
AU  - da Luz Martins M
LA  - eng
PT  - Journal Article
PL  - United States
TA  - Methods Mol Biol
JT  - Methods in molecular biology (Clifton, N.J.)
JID - 9214969
SB  - IM
MH  - AIDS-Related Opportunistic Infections/cerebrospinal fluid
MH  - Cerebrospinal Fluid/microbiology
MH  - Cryptococcus neoformans/cytology/*genetics/growth & development/*isolation & 
      purification
MH  - Culture Techniques
MH  - Humans
MH  - In Situ Hybridization, Fluorescence/*methods
EDAT- 2013/01/09 06:00
MHDA- 2013/06/07 06:00
CRDT- 2013/01/09 06:00
PHST- 2013/01/09 06:00 [entrez]
PHST- 2013/01/09 06:00 [pubmed]
PHST- 2013/06/07 06:00 [medline]
AID - 10.1007/978-1-62703-257-5_5 [doi]
PST - ppublish
SO  - Methods Mol Biol. 2013;968:71-82. doi: 10.1007/978-1-62703-257-5_5.