PMID- 23296981
OWN - NLM
STAT- MEDLINE
DCOM- 20130808
LR  - 20130319
IS  - 1479-6813 (Electronic)
IS  - 0952-5041 (Linking)
VI  - 50
IP  - 2
DP  - 2013 Apr
TI  - Septic shock non-thyroidal illness syndrome causes hypothyroidism and conditions 
      for reduced sensitivity to thyroid hormone.
PG  - 255-66
LID - 10.1530/JME-12-0188 [doi]
AB  - Non-thyroidal illness syndrome (NTIS) is part of the neuroendocrine response to 
      stress, but the significance of this syndrome remains uncertain. The aim of this 
      study was to investigate the effect of lipopolysaccharide (LPS)-induced NTIS on 
      thyroid hormone (TH) levels and TH molecular targets, as well as the relationship 
      between septic shock nuclear factor kappa-light-chain-enhancer of activated B 
      cells (NF-kB) activation and TH receptor beta (THRB) gene expression at a 
      multi-tissue level in a pig model. Prepubertal domestic pigs were given i.v. 
      saline or LPS for 48 h. Serum and tissue TH was measured by chemiluminescence and 
      RIA. Expression of THRs and cofactors was measured by real-time PCR, and 
      deiodinase (DIO) activity was measured by enzyme assays. Tissue NF-kB nuclear 
      binding activity was evaluated by EMSA. LPS-treated pigs had decreased TH levels 
      in serum and most tissues. DIO1 expression in liver and kidney and DIO1 activity 
      in kidney decreased after LPS. No changes in DIO2 activity were observed between 
      groups. LPS induced an increase in hypothalamus, thyroid, and liver DIO3 
      activity. Among the other studied genes, monocarboxylate transporter 8 and THRB 
      were the most commonly repressed in endotoxemic pigs. LPS-induced NF-kB 
      activation was associated with a decrease in THRB gene expression only in frontal 
      lobe, adrenal gland, and kidney cortex. We conclude that LPS-induced NTIS in pigs 
      is characterized by hypothyroidism and tissue-specific reduced TH sensitivity. 
      The role of NF-kB in regulating THRB expression during endotoxemia, if any, is 
      restricted to a limited number of tissues.
FAU - Castro, Isabel
AU  - Castro I
AD  - Division of Endocrinology, Department of Internal Medicine, Texas Tech University 
      Health Sciences Center School of Medicine, Lubbock, Texas 79430-9410, USA.
FAU - Quisenberry, Leah
AU  - Quisenberry L
FAU - Calvo, Rosa-Maria
AU  - Calvo RM
FAU - Obregon, Maria-Jesus
AU  - Obregon MJ
FAU - Lado-Abeal, Joaquin
AU  - Lado-Abeal J
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20130318
PL  - England
TA  - J Mol Endocrinol
JT  - Journal of molecular endocrinology
JID - 8902617
RN  - 0 (Lipopolysaccharides)
RN  - 0 (Monocarboxylic Acid Transporters)
RN  - 0 (NF-kappa B)
RN  - 0 (Receptors, Thyroid Hormone)
RN  - 0 (Thyroid Hormones)
RN  - EC 1.11.1.8 (Iodide Peroxidase)
SB  - IM
MH  - Animals
MH  - Enzyme Activation
MH  - Euthyroid Sick Syndromes/*complications/*etiology
MH  - Female
MH  - Gene Expression Regulation
MH  - Hypothyroidism/*etiology
MH  - Iodide Peroxidase/genetics/metabolism
MH  - Lipopolysaccharides/administration & dosage
MH  - Monocarboxylic Acid Transporters/genetics
MH  - NF-kappa B/metabolism
MH  - Receptors, Thyroid Hormone/genetics
MH  - Shock, Septic/*complications
MH  - Swine
MH  - Thyroid Hormones/blood/*metabolism
EDAT- 2013/01/09 06:00
MHDA- 2013/08/09 06:00
CRDT- 2013/01/09 06:00
PHST- 2013/01/09 06:00 [entrez]
PHST- 2013/01/09 06:00 [pubmed]
PHST- 2013/08/09 06:00 [medline]
AID - JME-12-0188 [pii]
AID - 10.1530/JME-12-0188 [doi]
PST - epublish
SO  - J Mol Endocrinol. 2013 Mar 18;50(2):255-66. doi: 10.1530/JME-12-0188. Print 2013 
      Apr.