PMID- 23297799
OWN - NLM
STAT- MEDLINE
DCOM- 20140224
LR  - 20190116
IS  - 1029-2403 (Electronic)
IS  - 1026-8022 (Linking)
VI  - 54
IP  - 8
DP  - 2013 Aug
TI  - Molecular characterization of neoplastic and normal "sister" lymphoblastoid 
      B-cell lines from chronic lymphocytic leukemia.
PG  - 1769-79
LID - 10.3109/10428194.2013.764418 [doi]
AB  - Chronic lymphocytic leukemia (CLL) B-cells resemble self-renewing CD5 + B-cells 
      carrying auto/xeno-antigen-reactive B-cell receptors (BCRs) and multiple innate 
      pattern-recognition receptors, such as Toll-like receptors and scavenger 
      receptors. Integration of signals from BCRs with multiple surface membrane 
      receptors determines whether the cells will be proliferating, anergic or 
      apoptotic. To better understand the role of antigen in leukemogenesis, CLL cell 
      lines producing monoclonal antibodies (mAbs) will facilitate structural analysis 
      of antigens and supply DNA for genetic studies. We present here a comprehensive 
      genotypic and phenotypic characterization of available CLL and normal 
      B-cell-derived lymphoblastoid cell lines (LCLs) from the same individuals (n = 
      17). Authenticity and verification studies of CLL-patient origin were done by 
      IGHV sequencing, fluorescence in situ hybridization (FISH) and DNA/short tandem 
      repeat (STR) fingerprinting. Innate B-cell features, i.e. natural Ab production 
      and CD5 receptors, were present in most CLL cell lines, but in none of the normal 
      LCLs. This panel of immortalized CLL-derived cell lines is a valuable reference 
      representing a renewable source of authentic Abs and DNA.
FAU - Lanemo Myhrinder, Anna
AU  - Lanemo Myhrinder A
AD  - Department of Clinical and Experimental Medicine, Linkoping University, 
      Linkoping, Sweden.
FAU - Hellqvist, Eva
AU  - Hellqvist E
FAU - Bergh, Ann-Charlotte
AU  - Bergh AC
FAU - Jansson, Mattias
AU  - Jansson M
FAU - Nilsson, Kenneth
AU  - Nilsson K
FAU - Hultman, Per
AU  - Hultman P
FAU - Jonasson, Jon
AU  - Jonasson J
FAU - Buhl, Anne Mette
AU  - Buhl AM
FAU - Bredo Pedersen, Lone
AU  - Bredo Pedersen L
FAU - Jurlander, Jesper
AU  - Jurlander J
FAU - Klein, Eva
AU  - Klein E
FAU - Weit, Nicole
AU  - Weit N
FAU - Herling, Marco
AU  - Herling M
FAU - Rosenquist, Richard
AU  - Rosenquist R
FAU - Rosen, Anders
AU  - Rosen A
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20130225
PL  - United States
TA  - Leuk Lymphoma
JT  - Leukemia & lymphoma
JID - 9007422
RN  - 0 (Antigens, Surface)
RN  - 0 (Complementarity Determining Regions)
RN  - 0 (Receptors, Antigen, B-Cell)
SB  - IM
MH  - Aged
MH  - Amino Acid Sequence
MH  - Antigens, Surface/metabolism
MH  - B-Lymphocytes/metabolism
MH  - Cell Line, Tumor
MH  - Complementarity Determining Regions/genetics
MH  - Female
MH  - Gene Expression Regulation, Leukemic
MH  - Gene Rearrangement, B-Lymphocyte
MH  - Gene Rearrangement, B-Lymphocyte, Heavy Chain
MH  - Humans
MH  - Immunophenotyping
MH  - Karyotype
MH  - Leukemia, Lymphocytic, Chronic, B-Cell/*genetics/*metabolism
MH  - Male
MH  - Microsatellite Repeats
MH  - Middle Aged
MH  - Molecular Sequence Data
MH  - Phenotype
MH  - Receptors, Antigen, B-Cell/genetics
MH  - Transcriptome
EDAT- 2013/01/10 06:00
MHDA- 2014/02/25 06:00
CRDT- 2013/01/10 06:00
PHST- 2013/01/10 06:00 [entrez]
PHST- 2013/01/10 06:00 [pubmed]
PHST- 2014/02/25 06:00 [medline]
AID - 10.3109/10428194.2013.764418 [doi]
PST - ppublish
SO  - Leuk Lymphoma. 2013 Aug;54(8):1769-79. doi: 10.3109/10428194.2013.764418. Epub 
      2013 Feb 25.