PMID- 23298344
OWN - NLM
STAT- MEDLINE
DCOM- 20130517
LR  - 20171116
IS  - 1365-3083 (Electronic)
IS  - 0300-9475 (Linking)
VI  - 77
IP  - 3
DP  - 2013 Mar
TI  - Age-matched dendritic cell subpopulations reference values in childhood.
PG  - 213-20
LID - 10.1111/sji.12024 [doi]
AB  - Dendritic cells (DCs) are the most potent antigen-presenting cells and are the 
      key link between the innate and adaptive immune response. Only a few reports with 
      study populations of up to 50 individuals have been published with age-based 
      reference values for DC subpopulations in healthy children. Therefore, we aimed 
      to establish reference ranges in a larger study population of 100 healthy 
      children, which allowed age-matched subgroups. Most previous studies were 
      performed using a dual-platform approach. In this study, a single-platform 
      approach in a lyse no-wash procedure was used. DC subpopulations were defined as 
      follows: CD45(+) CD85k(+) HLA-DR(+) CD14(-) CD16(-) CD33(+) cells as myeloid DCs 
      (mDCs) and CD45(+) CD85k(+) HLA-DR(+) CD14(-) CD16(-) CD123(+) cells as 
      plasmacytoid DCs (pDCs). Reference ranges were established using a 
      semi-parametric regression of age-matched absolute and relative DC counts. We 
      found a significant decline with increasing age in the medians of mDCs 
      (P = 0.0003) and pDCs per mul peripheral blood (PB) (P = 0.004) and in the 50%, 
      90% and 95% reference ranges. We also identified significantly lower absolute 
      cell counts of mDCs per mul PB in girls than in boys for all age groups 
      (P = 0.0015). Due to the larger paediatric study population and single-platform 
      approach, this study may give a more precise overview of the normal age-matched 
      development of DC subpopulations and may provide a basis for analyzing abnormal 
      DC counts in different illnesses or therapies such as post stem cell 
      transplantation.
CI  - (c) 2013 The Authors. Scandinavian Journal of Immunology (c) 2013 Blackwell 
      Publishing Ltd.
FAU - Heinze, A
AU  - Heinze A
AD  - Department of Pediatrics, Johann Wolfgang Goethe-University Hospital, Frankfurt 
      am Main, Germany. annekathrin.heinze@kgu.de
FAU - Elze, M C
AU  - Elze MC
FAU - Kloess, S
AU  - Kloess S
FAU - Ciocarlie, O
AU  - Ciocarlie O
FAU - Konigs, C
AU  - Konigs C
FAU - Betz, S
AU  - Betz S
FAU - Bremm, M
AU  - Bremm M
FAU - Esser, R
AU  - Esser R
FAU - Klingebiel, T
AU  - Klingebiel T
FAU - Serban, M
AU  - Serban M
FAU - Hutton, J L
AU  - Hutton JL
FAU - Koehl, U
AU  - Koehl U
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - England
TA  - Scand J Immunol
JT  - Scandinavian journal of immunology
JID - 0323767
RN  - 0 (Antigens, CD)
RN  - 0 (HLA-DR Antigens)
RN  - 0 (Interleukin-3 Receptor alpha Subunit)
RN  - 0 (LILRB1 protein, human)
RN  - 0 (Leukocyte Immunoglobulin-like Receptor B1)
RN  - 0 (Lipopolysaccharide Receptors)
RN  - 0 (Receptors, IgG)
RN  - 0 (Receptors, Immunologic)
RN  - EC 3.1.3.48 (Leukocyte Common Antigens)
SB  - IM
MH  - Adolescent
MH  - Age Factors
MH  - Antigens, CD/immunology/metabolism
MH  - Cell Count
MH  - Child
MH  - Child, Preschool
MH  - Dendritic Cells/*cytology/*immunology/metabolism
MH  - Female
MH  - Flow Cytometry
MH  - HLA-DR Antigens/immunology/metabolism
MH  - Humans
MH  - Infant
MH  - Infant, Newborn
MH  - Interleukin-3 Receptor alpha Subunit/immunology/metabolism
MH  - Leukocyte Common Antigens/immunology/metabolism
MH  - Leukocyte Immunoglobulin-like Receptor B1
MH  - Lipopolysaccharide Receptors/immunology/metabolism
MH  - Male
MH  - Myeloid Cells/cytology/immunology/metabolism
MH  - Receptors, IgG/immunology/metabolism
MH  - Receptors, Immunologic/immunology/metabolism
MH  - Regression Analysis
MH  - Sex Factors
EDAT- 2013/01/10 06:00
MHDA- 2013/05/18 06:00
CRDT- 2013/01/10 06:00
PHST- 2012/07/19 00:00 [received]
PHST- 2012/12/25 00:00 [accepted]
PHST- 2013/01/10 06:00 [entrez]
PHST- 2013/01/10 06:00 [pubmed]
PHST- 2013/05/18 06:00 [medline]
AID - 10.1111/sji.12024 [doi]
PST - ppublish
SO  - Scand J Immunol. 2013 Mar;77(3):213-20. doi: 10.1111/sji.12024.