PMID- 23300658
OWN - NLM
STAT- MEDLINE
DCOM- 20130702
LR  - 20220321
IS  - 1932-6203 (Electronic)
IS  - 1932-6203 (Linking)
VI  - 7
IP  - 12
DP  - 2012
TI  - A novel rabbit immunospot array assay on a chip allows for the rapid generation 
      of rabbit monoclonal antibodies with high affinity.
PG  - e52383
LID - 10.1371/journal.pone.0052383 [doi]
LID - e52383
AB  - Antigen-specific rabbit monoclonal antibodies (RaMoAbs) are useful due to their 
      high specificity and high affinity, and the establishment of a comprehensive and 
      rapid RaMoAb generation system has been highly anticipated. Here, we present a 
      novel system using immunospot array assay on a chip (ISAAC) technology in which 
      we detect and retrieve antigen-specific antibody-secreting cells from the 
      peripheral blood lymphocytes of antigen-immunized rabbits and produce 
      antigen-specific RaMoAbs with 10(-12) M affinity within a time period of only 7 
      days. We have used this system to efficiently generate RaMoAbs that are specific 
      to a phosphorylated signal-transducing molecule. Our system provides a new method 
      for the comprehensive and rapid production of RaMoAbs, which may contribute to 
      laboratory research and clinical applications.
FAU - Ozawa, Tatsuhiko
AU  - Ozawa T
AD  - Department of Immunology, Graduate School of Medicine and Pharmaceutical 
      Sciences, University of Toyama, Toyama, Japan.
FAU - Piao, Xiuhong
AU  - Piao X
FAU - Kobayashi, Eiji
AU  - Kobayashi E
FAU - Zhou, Yue
AU  - Zhou Y
FAU - Sakurai, Hiroaki
AU  - Sakurai H
FAU - Andoh, Tsugunobu
AU  - Andoh T
FAU - Jin, Aishun
AU  - Jin A
FAU - Kishi, Hiroyuki
AU  - Kishi H
FAU - Muraguchi, Atsushi
AU  - Muraguchi A
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20121226
PL  - United States
TA  - PLoS One
JT  - PloS one
JID - 101285081
RN  - 0 (Antibodies, Monoclonal)
RN  - 0 (Peptides)
SB  - IM
MH  - Animals
MH  - Antibodies, Monoclonal/biosynthesis/*immunology
MH  - *Antibody Affinity
MH  - Antibody Specificity
MH  - B-Lymphocytes/immunology
MH  - Humans
MH  - Immunoassay/*methods
MH  - Microarray Analysis/*methods
MH  - Peptides/immunology/metabolism
MH  - Phosphorylation
MH  - Rabbits
MH  - Signal Transduction
MH  - Time Factors
PMC - PMC3530603
COIS- Competing Interests: AM was supported by a research fund from Vivalis (Nantes, 
      France.) The ISAAC method is covered by patents that have been exclusively 
      licensed to Vivalis (Nantes, France.) Patent details: Pub.No.: WO/2009/017226, 
      International Application No.: PCT/JP2008/063874, Title: CELL SCREENING METHOD; 
      HK is one of Scientific Advisory Board Members of Vivalis. Microwell array chips 
      are now provided to us by Vivalis. There are no further patents, products in 
      development or marketed products to declare. This does not alter the authors' 
      adherence to all the PLOS ONE policies on sharing data and materials, as detailed 
      online in the guide for authors.
EDAT- 2013/01/10 06:00
MHDA- 2013/07/03 06:00
PMCR- 2012/12/26
CRDT- 2013/01/10 06:00
PHST- 2012/07/31 00:00 [received]
PHST- 2012/11/12 00:00 [accepted]
PHST- 2013/01/10 06:00 [entrez]
PHST- 2013/01/10 06:00 [pubmed]
PHST- 2013/07/03 06:00 [medline]
PHST- 2012/12/26 00:00 [pmc-release]
AID - PONE-D-12-22543 [pii]
AID - 10.1371/journal.pone.0052383 [doi]
PST - ppublish
SO  - PLoS One. 2012;7(12):e52383. doi: 10.1371/journal.pone.0052383. Epub 2012 Dec 26.