PMID- 23301028
OWN - NLM
STAT- MEDLINE
DCOM- 20130820
LR  - 20211021
IS  - 1932-6203 (Electronic)
IS  - 1932-6203 (Linking)
VI  - 8
IP  - 1
DP  - 2013
TI  - Poly(dimethylsiloxane) (PDMS) affects gene expression in PC12 cells 
      differentiating into neuronal-like cells.
PG  - e53107
LID - 10.1371/journal.pone.0053107 [doi]
LID - e53107
AB  - INTRODUCTION: Microfluidics systems usually consist of materials like 
      PMMA--poly(methyl methacrylate) and PDMS--poly(dimethylsiloxane) and not 
      polystyrene (PS), which is usually used for cell culture. Cellular and molecular 
      responses in cells grown on PS are well characterized due to decades of 
      accumulated research. In contrast, the experience base is limited for materials 
      used in microfludics chip fabrication. METHODS: The effect of different materials 
      (PS, PMMA and perforated PMMA with a piece of PDMS underneath) on the growth and 
      differentiation of PC12 (adrenal phaeochromocytoma) cells into neuronal-like 
      cells was investigated using cell viability, cell cycle distribution, morphology, 
      and gene expression analysis. RESULTS/CONCLUSIONS: After differentiation, the 
      morphology, viability and cell cycle distribution of PC12 cells grown on PS, PMMA 
      with and without PDMS underneath was the same. By contrast, 41 genes showed 
      different expression for PC12 cells differentiating on PMMA as compared to on PS. 
      In contrast, 677 genes showed different expression on PMMA with PDMS underneath 
      as compared with PC12 cells on PS. The differentially expressed genes are 
      involved in neuronal cell development and function. However, there were also many 
      markers for neuronal cell development and functions that were expressed similarly 
      in cells differentiating on PS, PMMA and PMMA with PDMS underneath. In 
      conclusion, it was shown that PMMA has a minor impact and PDMS a major impact on 
      gene expression in PC12 cells.
FAU - Lopacinska, Joanna M
AU  - Lopacinska JM
AD  - Department of Micro- and Nanotechnology, Technical University of Denmark, Kongens 
      Lyngby, Denmark.
FAU - Emneus, Jenny
AU  - Emneus J
FAU - Dufva, Martin
AU  - Dufva M
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20130103
PL  - United States
TA  - PLoS One
JT  - PloS one
JID - 101285081
RN  - 0 (Biocompatible Materials)
RN  - 0 (Dimethylpolysiloxanes)
RN  - 0 (Polystyrenes)
RN  - 63148-62-9 (baysilon)
RN  - 9011-14-7 (Polymethyl Methacrylate)
SB  - IM
MH  - Animals
MH  - Biocompatible Materials
MH  - Cell Culture Techniques
MH  - Cell Cycle
MH  - Cell Survival
MH  - Dimethylpolysiloxanes/*chemistry
MH  - Equipment Design
MH  - G1 Phase
MH  - Gene Expression/*drug effects
MH  - Gene Expression Profiling
MH  - Microfluidic Analytical Techniques/instrumentation
MH  - Microfluidics/*instrumentation
MH  - Neurons/*metabolism
MH  - Oligonucleotide Array Sequence Analysis
MH  - PC12 Cells
MH  - Polymethyl Methacrylate/chemistry
MH  - Polystyrenes/chemistry
MH  - Predictive Value of Tests
MH  - Rats
PMC - PMC3536795
COIS- Competing Interests: The authors have declared that no competing interests exist.
EDAT- 2013/01/10 06:00
MHDA- 2013/08/21 06:00
PMCR- 2013/01/03
CRDT- 2013/01/10 06:00
PHST- 2012/07/31 00:00 [received]
PHST- 2012/11/23 00:00 [accepted]
PHST- 2013/01/10 06:00 [entrez]
PHST- 2013/01/10 06:00 [pubmed]
PHST- 2013/08/21 06:00 [medline]
PHST- 2013/01/03 00:00 [pmc-release]
AID - PONE-D-12-23126 [pii]
AID - 10.1371/journal.pone.0053107 [doi]
PST - ppublish
SO  - PLoS One. 2013;8(1):e53107. doi: 10.1371/journal.pone.0053107. Epub 2013 Jan 3.