PMID- 23301942
OWN - NLM
STAT- MEDLINE
DCOM- 20140626
LR  - 20211021
IS  - 1563-5279 (Electronic)
IS  - 0020-7454 (Print)
IS  - 0020-7454 (Linking)
VI  - 123
IP  - 7
DP  - 2013 Jul
TI  - Changes in responsiveness of rat spiral ganglion neurons to neurotrophins across 
      age: differential regulation of survival and neuritogenesis.
PG  - 465-75
LID - 10.3109/00207454.2013.764497 [doi]
AB  - Developmental changes in responsiveness of rat spiral ganglion neurons (SGNs) to 
      neurotrophin-3 (NT-3) and brain-derived neurotrophic factor (BDNF) were examined 
      using an explant culture system. Spiral ganglion (SG) explants at embryonic Day 
      18 (E18), postnatal Day 0 (P0), P5, P10 and P20 were cultured with the addition 
      of either NT-3 or BDNF at various concentrations (0.1-100 ng/ml) and analyzed the 
      dose-response characteristics of three parameters: SGN survival, the number of 
      neurites emanating from the explants and the length of neurite extension. In E18 
      cultures, SGN survival and neurite number were enhanced more strongly by NT-3 
      than by the BDNF. As the explants became more mature, the effects of NT-3 
      decreased, whereas those of BDNF increased, peaking at P0. Although the intrinsic 
      capacity of SGNs to produce and extend neurites declined considerably by P20, 
      they still retained the capacity to respond to both NT-3 and BDNF. These temporal 
      patterns in responsiveness of SGNs to neurotrophins correspond well to the 
      expression pattern of the two neurotrophins in cochlear sensory epithelium in 
      vivo and also correlate with the time course of developmental events in SGNs such 
      as cell death and the establishment of mature hair cell innervation patterns.
FAU - Kondo, Kenji
AU  - Kondo K
AD  - Division of Otolaryngology and Neurosciences, Department of Surgery and Veterans 
      Administration Medical Center, University of California, San Diego School of 
      Medicine, La Jolla, CA 92093, USA.
FAU - Pak, Kwang
AU  - Pak K
FAU - Chavez, Eduardo
AU  - Chavez E
FAU - Mullen, Lina
AU  - Mullen L
FAU - Euteneuer, Sara
AU  - Euteneuer S
FAU - Ryan, Allen F
AU  - Ryan AF
LA  - eng
GR  - R01 DC000139/DC/NIDCD NIH HHS/United States
GR  - DC000139/DC/NIDCD NIH HHS/United States
PT  - Journal Article
PT  - Research Support, N.I.H., Extramural
PT  - Research Support, Non-U.S. Gov't
PT  - Research Support, U.S. Gov't, Non-P.H.S.
DEP - 20130211
PL  - England
TA  - Int J Neurosci
JT  - The International journal of neuroscience
JID - 0270707
RN  - 0 (Brain-Derived Neurotrophic Factor)
RN  - 0 (Neurotrophin 3)
RN  - 0 (Receptors, Nerve Growth Factor)
SB  - IM
MH  - Aging/*physiology
MH  - Animals
MH  - Apoptosis/drug effects
MH  - Brain-Derived Neurotrophic Factor/pharmacology/*physiology
MH  - Cell Survival/drug effects
MH  - Cells, Cultured
MH  - Dose-Response Relationship, Drug
MH  - Neurites/*drug effects
MH  - Neurons/cytology/*drug effects/*physiology
MH  - Neurotrophin 3/pharmacology/*physiology
MH  - Rats
MH  - Receptors, Nerve Growth Factor/biosynthesis
MH  - Spiral Ganglion/*cytology
PMC - PMC3897193
MID - NIHMS539777
EDAT- 2013/01/11 06:00
MHDA- 2014/06/27 06:00
PMCR- 2014/01/21
CRDT- 2013/01/11 06:00
PHST- 2013/01/11 06:00 [entrez]
PHST- 2013/01/11 06:00 [pubmed]
PHST- 2014/06/27 06:00 [medline]
PHST- 2014/01/21 00:00 [pmc-release]
AID - 10.3109/00207454.2013.764497 [doi]
PST - ppublish
SO  - Int J Neurosci. 2013 Jul;123(7):465-75. doi: 10.3109/00207454.2013.764497. Epub 
      2013 Feb 11.