PMID- 23302384
OWN - NLM
STAT- MEDLINE
DCOM- 20140428
LR  - 20181202
IS  - 1002-0098 (Print)
IS  - 1002-0098 (Linking)
VI  - 47
IP  - 10
DP  - 2012 Oct
TI  - [Effect of berberine hydrochloride on the secretion of monocyte chemoattractant 
      protein-1 from human periodontal ligament cells in vitro].
PG  - 610-3
LID - 10.3760/cma.j.issn.1002-0098.2012.10.008 [doi]
AB  - OBJECTIVE: To observe the effects of berberine hydrochloride on the secretion of 
      monocyte chemoattractant protein-1 (MCP-1) from human periodontal ligament cells 
      (PDLC) in vitro culture. METHODS: Periodontal ligament was isolated from 
      extracted human premolars, and PDLC were cultured in vitro. PDLC were divided 
      into two groups, lipopolysaccharide (LPS) group and non-lipopolysaccharide (NLPS) 
      group. Then in accordance with the final concentrations of berberine 
      hydrochloride in cells culture medium (0, 0.01, 0.02, 0.03 g/L), the groups were 
      subdivided into LPS and NLPS control group, LPS1 and NLPS1 group, LPS2 and NLPS2 
      group, LPS3 and NLPS3 group. Cellular concentration of MCP-1 of each group was 
      determined by enzyme-linked immunosorbent assay (ELISA). The data were 
      statistically analyzed. RESULTS: The MCP-1 contents were not significantly 
      different between the groups of NLPS1, NLPS2 and NLPS3 [(11.33 +/- 0.16), (11.45 +/- 
      0.53), (11.25 +/- 0.14) ng/L, respectively] and the NLPS control group [(11.32 +/- 
      0.35) ng/L] (P = 0.692, 0.568, 0.524). MCP-1 contents in the groups of LPS1, LPS2 
      and LPS3 [respectively (38.14 +/- 5.34), (34.15 +/- 3.36), (26.13 +/- 2.12) ng/L] were 
      significantly lower than in LPS control group [(58.42 +/- 1.52) ng/L], P = 0.000, 
      0.000, P = 0.000. CONCLUSIONS: The inhibitory effect of berberine hydrochloride 
      on the activities of MCP-1 from PDLC was more significant when PDLC were 
      stimulated with LPS and in a concentration-dependent manner.
FAU - Zhang, Fan
AU  - Zhang F
AD  - Department of Prosthodontics, School of Stomatology, Lanzhou University, Lanzhou 
      730000, China. yuzhanhai@lzu.edu.cn
FAU - Yu, Zhan-hai
AU  - Yu ZH
LA  - chi
PT  - Journal Article
PL  - China
TA  - Zhonghua Kou Qiang Yi Xue Za Zhi
JT  - Zhonghua kou qiang yi xue za zhi = Zhonghua kouqiang yixue zazhi = Chinese 
      journal of stomatology
JID - 8711066
RN  - 0 (Anti-Inflammatory Agents)
RN  - 0 (CCL2 protein, human)
RN  - 0 (Chemokine CCL2)
RN  - 0 (Lipopolysaccharides)
RN  - 0I8Y3P32UF (Berberine)
SB  - IM
MH  - Adolescent
MH  - Anti-Inflammatory Agents/administration & dosage/*pharmacology
MH  - Berberine/administration & dosage/*pharmacology
MH  - Bicuspid/cytology
MH  - Cells, Cultured
MH  - Chemokine CCL2/*metabolism
MH  - Child
MH  - Dose-Response Relationship, Drug
MH  - Humans
MH  - Lipopolysaccharides/pharmacology
MH  - Periodontal Ligament/cytology/*metabolism
EDAT- 2013/01/11 06:00
MHDA- 2014/04/29 06:00
CRDT- 2013/01/11 06:00
PHST- 2013/01/11 06:00 [entrez]
PHST- 2013/01/11 06:00 [pubmed]
PHST- 2014/04/29 06:00 [medline]
AID - 10.3760/cma.j.issn.1002-0098.2012.10.008 [doi]
PST - ppublish
SO  - Zhonghua Kou Qiang Yi Xue Za Zhi. 2012 Oct;47(10):610-3. doi: 
      10.3760/cma.j.issn.1002-0098.2012.10.008.