PMID- 23302599
OWN - NLM
STAT- MEDLINE
DCOM- 20150115
LR  - 20140303
IS  - 1875-8622 (Electronic)
IS  - 1386-0291 (Linking)
VI  - 56
IP  - 1
DP  - 2014
TI  - Transforming growth factor-beta1 deteriorates microrheological characteristics and 
      motility of mature dendritic cells in concentration-dependent fashion.
PG  - 25-40
LID - 10.3233/CH-121653 [doi]
AB  - Dendritic cells (DCs) are potent and specialized antigen-presenting cells that 
      play a crucial role in initiating and amplifying both the innate and adaptive 
      immune responses. Tumor cells can escape from immune attack by secreting 
      suppressive cytokines which solely or cooperatively impair the immune function 
      and microrheological properties of DCs. However, the underlying mechanisms are 
      not fully defined. Transforming growth factor-beta1 (TGF-beta1) has been identified as 
      a major cytokine in the tumor microenvironment. To determine the effects of 
      TGF-beta1 on mature DCs (mDCs) from microrheological viewpoint, cells were treated 
      with different concentrations of TGF-beta1. The results showed that the impaired 
      microrheological parameters, including osmotic fragility, electrophoretic 
      mobility, deformability, membrane fluidity, F-actin organization and so on, as 
      well as motilities of mDCs relied heavily on TGF-beta1 concentration. Moreover, 
      these changes were correlated with the expression levels of fascin1, cofilin1, 
      phosphorylated cofilin1 and profilin, this could be one of the crucial aspects of 
      immune escape mechanisms of tumors, hinting that the signal pathway of TGF-beta1 
      should be blocked in appropriate way before performing DCs-based immunotherapy 
      against cancer. It is clinically important to understand the biological behavior 
      of DCs and immune escape mechanism of tumor as well as how to improve efficiency 
      of the anti-tumor therapy based on DCs.
FAU - Zheng, Qinni
AU  - Zheng Q
AD  - Department of Biotechnology, Guiyang Medical College, Guiyang, Guizhou Province, 
      China Department of Cell Biology, Guiyang Medical College, Guiyang, Guizhou 
      Province, China.
FAU - Long, Jinhua
AU  - Long J
AD  - Department of Head and Neck, Affiliated Hospital and Cancer Hospital, Guiyang 
      Medical College, Guiyang, Guizhou Province, China.
FAU - Jia, Binbin
AU  - Jia B
AD  - Department of Medical Physics, Chengde Medical College, Chengde, Hebei Province, 
      China.
FAU - Xu, Xiaoli
AU  - Xu X
AD  - Department of Biotechnology, Guiyang Medical College, Guiyang, Guizhou Province, 
      China Department of Cell Biology, Guiyang Medical College, Guiyang, Guizhou 
      Province, China.
FAU - Zhang, Chunlin
AU  - Zhang C
AD  - Department of Cell Biology, Guiyang Medical College, Guiyang, Guizhou Province, 
      China.
FAU - Li, Long
AU  - Li L
AD  - Department of Nephrology, Affiliated Hospital of Guiyang Medical College, 
      Guiyang, Guizhou Province, China.
FAU - Wen, Zongyao
AU  - Wen Z
AD  - Hemorheology Center, School of Basic Medical Sciences, Health Science Center of 
      Peking University, Beijing, China.
FAU - Jin, Feng
AU  - Jin F
AD  - Department of Head and Neck, Affiliated Hospital and Cancer Hospital, Guiyang 
      Medical College, Guiyang, Guizhou Province, China.
FAU - Yao, Weijuan
AU  - Yao W
AD  - Hemorheology Center, School of Basic Medical Sciences, Health Science Center of 
      Peking University, Beijing, China.
FAU - Zeng, Zhu
AU  - Zeng Z
AD  - Department of Biotechnology, Guiyang Medical College, Guiyang, Guizhou Province, 
      China Department of Cell Biology, Guiyang Medical College, Guiyang, Guizhou 
      Province, China.
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - Netherlands
TA  - Clin Hemorheol Microcirc
JT  - Clinical hemorheology and microcirculation
JID - 9709206
RN  - 0 (Actins)
RN  - 0 (Antigens, CD)
RN  - 0 (Transforming Growth Factor beta1)
RN  - 0 (Viscoelastic Substances)
SB  - IM
MH  - Actins/metabolism
MH  - Antigens, CD/blood
MH  - Apoptosis/drug effects
MH  - Dendritic Cells/cytology/*drug effects/metabolism
MH  - Human Umbilical Vein Endothelial Cells
MH  - Humans
MH  - Linear Models
MH  - Microscopy, Confocal
MH  - Osmotic Fragility
MH  - Rheology/methods
MH  - Transforming Growth Factor beta1/*pharmacology
MH  - Viscoelastic Substances/chemistry
OTO - NOTNLM
OT  - Mature dendritic cells
OT  - immune escape mechanism of tumor
OT  - microrheological characteristics
OT  - transforming growth factor-$\beta_1$
EDAT- 2013/01/11 06:00
MHDA- 2015/01/16 06:00
CRDT- 2013/01/11 06:00
PHST- 2013/01/11 06:00 [entrez]
PHST- 2013/01/11 06:00 [pubmed]
PHST- 2015/01/16 06:00 [medline]
AID - 34925745V2713828 [pii]
AID - 10.3233/CH-121653 [doi]
PST - ppublish
SO  - Clin Hemorheol Microcirc. 2014;56(1):25-40. doi: 10.3233/CH-121653.