PMID- 23303496
OWN - NLM
STAT- MEDLINE
DCOM- 20130729
LR  - 20211021
IS  - 1098-660X (Electronic)
IS  - 0095-1137 (Print)
IS  - 0095-1137 (Linking)
VI  - 51
IP  - 3
DP  - 2013 Mar
TI  - Single-tube multiplexed molecular detection of endemic porcine viruses in 
      combination with background screening for transboundary diseases.
PG  - 938-44
LID - 10.1128/JCM.02947-12 [doi]
AB  - Detection of several pathogens with multiplexed real-time quantitative PCR (qPCR) 
      assays in a one-step setup allows the simultaneous detection of two endemic 
      porcine and four different selected transboundary viruses. Reverse transcription 
      (RT)-qPCR systems for the detection of porcine reproductive and respiratory 
      syndrome virus (PRRSV) and porcine circovirus type 2 (PCV2), two of the most 
      economically important pathogens of swine worldwide, were combined with a 
      screening system for diseases notifiable to the World Organization of Animal 
      Health, namely, classical and African swine fever, foot-and-mouth disease, and 
      Aujeszky's disease. Background screening was implemented using the identical 
      fluorophore for all four different RT-qPCR assays. The novel multiplex RT-qPCR 
      system was validated with a large panel of different body fluids and tissues from 
      pigs and other animal species. Both reference samples and clinical specimens were 
      used for a complete evaluation. It could be demonstrated that a highly sensitive 
      and specific parallel detection of the different viruses was possible. The assays 
      for the notifiable diseases were even not affected by the simultaneous 
      amplification of very high loads of PRRSV- and PCV2-specific sequences. The novel 
      broad-spectrum multiplex assay allows in a unique form the routine investigation 
      for endemic porcine pathogens with exclusion diagnostics of the most important 
      transboundary diseases in samples from pigs with unspecific clinical signs, such 
      as fever or hemorrhages. The new system could significantly improve early 
      detection of the most important notifiable diseases of swine and could lead to a 
      new approach in syndromic surveillance.
FAU - Wernike, Kerstin
AU  - Wernike K
AD  - Institute of Diagnostic Virology, Friedrich-Loeffler-Institut (FLI), 
      Greifswald-Insel Riems, Germany.
FAU - Hoffmann, Bernd
AU  - Hoffmann B
FAU - Beer, Martin
AU  - Beer M
LA  - eng
PT  - Evaluation Study
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20130109
PL  - United States
TA  - J Clin Microbiol
JT  - Journal of clinical microbiology
JID - 7505564
SB  - IM
MH  - Animals
MH  - Multiplex Polymerase Chain Reaction/*methods
MH  - Real-Time Polymerase Chain Reaction/*methods
MH  - Reverse Transcriptase Polymerase Chain Reaction/methods
MH  - Sensitivity and Specificity
MH  - Swine
MH  - Swine Diseases/*diagnosis/virology
MH  - Veterinary Medicine/*methods
MH  - Virology/*methods
MH  - Virus Diseases/diagnosis/*veterinary/virology
MH  - Viruses/classification/genetics/*isolation & purification
PMC - PMC3592051
EDAT- 2013/01/11 06:00
MHDA- 2013/07/31 06:00
PMCR- 2013/09/01
CRDT- 2013/01/11 06:00
PHST- 2013/01/11 06:00 [entrez]
PHST- 2013/01/11 06:00 [pubmed]
PHST- 2013/07/31 06:00 [medline]
PHST- 2013/09/01 00:00 [pmc-release]
AID - JCM.02947-12 [pii]
AID - 02947-12 [pii]
AID - 10.1128/JCM.02947-12 [doi]
PST - ppublish
SO  - J Clin Microbiol. 2013 Mar;51(3):938-44. doi: 10.1128/JCM.02947-12. Epub 2013 Jan 
      9.