PMID- 23303806
OWN - NLM
STAT- MEDLINE
DCOM- 20130418
LR  - 20231213
IS  - 1537-6613 (Electronic)
IS  - 0022-1899 (Print)
IS  - 0022-1899 (Linking)
VI  - 207
IP  - 7
DP  - 2013 Apr
TI  - Role of the interleukin 10 family of cytokines in patients with immune 
      reconstitution inflammatory syndrome associated with HIV infection and 
      tuberculosis.
PG  - 1148-56
LID - 10.1093/infdis/jit002 [doi]
AB  - BACKGROUND: The interleukin 10 (IL-10) family comprises cytokines structurally 
      related to IL-10 that share signaling receptors that have conserved signaling 
      cascades. The immunopathogenesis of immune reconstitution inflammatory syndrome 
      (IRIS) in patients with human immunodeficiency virus (HIV) infection and 
      tuberculosis remains incompletely understood. We hypothesized that a deficiency 
      of IL-10 and its homologs may contribute to the immunopathology of IRIS in these 
      patients. METHODS: We performed a case-control analysis involving patients with 
      HIV infection and tuberculosis who had IRIS at clinical presentation 
      (tuberculosis-IRIS) and similar patients with HIV infection and tuberculosis who 
      did not develop tuberculosis-IRIS (non-IRIS). Peripheral blood mononuclear cells 
      (PBMCs) were cultured in the presence or absence of heat-killed Mycobacterium 
      tuberculosis for 6 and 24 hours. Messenger RNA was analyzed by quantitative 
      reverse transcription polymerase chain reaction analysis. Cytokine concentrations 
      in serum were also determined. RESULTS: Cultures of PBMCs stimulated with M. 
      tuberculosis for 24 hours yielded higher IL-10 and interleukin 22 (IL-22) 
      transcript levels for tuberculosis-IRIS patients, compared with non-IRIS 
      patients. Analysis of corresponding serum samples showed significantly higher 
      concentrations of IL-10 and IL-22 in tuberculosis-IRIS patients, compared with 
      non-IRIS patients. CONCLUSIONS: IL-10 and IL-22 were differentially induced in 
      PBMCs from tuberculosis-IRIS patients after in vitro stimulation, and higher 
      concentrations of their corresponding proteins were detected in serum (in vivo). 
      The higher levels of IL-10 observed in this study may represent a compensatory 
      antiinflammatory response during tuberculosis-IRIS. The elevated levels of IL-22 
      suggest an association between this cytokine and immunopathology during 
      tuberculosis-IRIS.
FAU - Tadokera, Rebecca
AU  - Tadokera R
AD  - Clinical Infectious Diseases Research Initiative, Institute of Infectious 
      Diseases and Molecular Medicine, University of Cape Town, Cape Town, South 
      Africa.
FAU - Wilkinson, Katalin A
AU  - Wilkinson KA
FAU - Meintjes, Graeme A
AU  - Meintjes GA
FAU - Skolimowska, Keira H
AU  - Skolimowska KH
FAU - Matthews, Kerryn
AU  - Matthews K
FAU - Seldon, Ronnett
AU  - Seldon R
FAU - Rangaka, Molebogeng X
AU  - Rangaka MX
FAU - Maartens, Gary
AU  - Maartens G
FAU - Wilkinson, Robert J
AU  - Wilkinson RJ
LA  - eng
GR  - U1175.02.002.00014.01/MRC_/Medical Research Council/United Kingdom
GR  - 081667/WT_/Wellcome Trust/United Kingdom
GR  - 088316/Wellcome Trust/United Kingdom
GR  - 084323/Wellcome Trust/United Kingdom
GR  - MC_U117588499/MRC_/Medical Research Council/United Kingdom
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20130109
PL  - United States
TA  - J Infect Dis
JT  - The Journal of infectious diseases
JID - 0413675
RN  - 0 (IL10 protein, human)
RN  - 0 (Interleukins)
RN  - 0 (RNA, Messenger)
RN  - 130068-27-8 (Interleukin-10)
SB  - IM
MH  - Adult
MH  - CD4 Lymphocyte Count
MH  - Case-Control Studies
MH  - Cells, Cultured
MH  - Cross-Sectional Studies
MH  - Female
MH  - HIV/immunology/*pathogenicity
MH  - HIV Infections/immunology/*pathology
MH  - Humans
MH  - Immune Reconstitution Inflammatory 
      Syndrome/immunology/microbiology/*pathology/virology
MH  - Interleukin-10/blood/genetics/*immunology
MH  - Interleukins/blood/genetics/immunology
MH  - Leukocytes, Mononuclear/immunology/microbiology/virology
MH  - Male
MH  - Middle Aged
MH  - Mycobacterium tuberculosis/immunology/pathogenicity
MH  - RNA, Messenger/analysis/genetics
MH  - Reverse Transcriptase Polymerase Chain Reaction/methods
MH  - Time Factors
MH  - Tuberculosis/immunology/*pathology
MH  - Young Adult
MH  - Interleukin-22
PMC - PMC3583273
EDAT- 2013/01/11 06:00
MHDA- 2013/04/20 06:00
PMCR- 2013/01/09
CRDT- 2013/01/11 06:00
PHST- 2013/01/11 06:00 [entrez]
PHST- 2013/01/11 06:00 [pubmed]
PHST- 2013/04/20 06:00 [medline]
PHST- 2013/01/09 00:00 [pmc-release]
AID - jit002 [pii]
AID - 10.1093/infdis/jit002 [doi]
PST - ppublish
SO  - J Infect Dis. 2013 Apr;207(7):1148-56. doi: 10.1093/infdis/jit002. Epub 2013 Jan 
      9.