PMID- 23305248
OWN - NLM
STAT- MEDLINE
DCOM- 20131112
LR  - 20130910
IS  - 1537-2995 (Electronic)
IS  - 0041-1132 (Linking)
VI  - 53
IP  - 9
DP  - 2013 Sep
TI  - Virally inactivated human platelet concentrate lysate induces regulatory T cells 
      and immunosuppressive effect in a murine asthma model.
PG  - 1918-28
LID - 10.1111/trf.12068 [doi]
AB  - BACKGROUND: Platelet concentrate lysates (PCLs) are increasingly used in 
      regenerative medicine. We have developed a solvent/detergent (S/D)-treated PCL. 
      The functional properties of this preparation should be unveiled. We hypothesized 
      that, due to transforming growth factor-beta1 (TGF-beta1) content, PCLs may exert 
      immunosuppressive and anti-inflammatory functions. STUDY DESIGN AND METHODS: PCL 
      was prepared by S/D treatment, oil extraction, and hydrophobic interaction 
      chromatography. The content of TGF-beta in PCL was determined by enzyme-linked 
      immunosorbent assay. Cultured CD4+ T cells were used to investigate the effects 
      of PCL on expression of transcription factor forkhead box P3 (Foxp3), the 
      inhibition of T-cell proliferation, and cytokine production. The regulatory 
      function of PCL-converted CD4+ T cells was analyzed by suppressive assay. The 
      BALB/c mice were given PCL-converted CD4+ T cells before ovalbumin (OVA) 
      sensitization and challenge using an asthma model. Inflammatory parameters, such 
      as the level of immunoglobulin E (IgE), airway hyperresponsiveness (AHR), 
      bronchial lavage fluid eosinophils, and cytokines were assayed. Recombinant human 
      (rHu) TGF-beta1 was used as control. RESULTS: PCL significantly enhanced the 
      development of CD4+Foxp3+-induced regulatory T cells (iTregs). Converted iTregs 
      produced neither Th1 nor Th2 cytokines and inhibited normal T-cell proliferation. 
      PCL- and rHuTGF-beta-converted CD4+ T cells prevented OVA-induced asthma. PCL- and 
      rHuTGF-beta-modified T cells both significantly reduced expression levels of 
      OVA-specific IgE and significantly inhibited the development of AHR, airway 
      eosinophilia, and Th2 responses in mice. CONCLUSION: S/D-treated PCL promotes 
      Foxp3+ iTregs and exerts immunosuppressive and anti-inflammatory properties. This 
      finding may help to understand the clinical properties of platelet lysates.
CI  - (c) 2013 American Association of Blood Banks.
FAU - Lee, Yueh-Lun
AU  - Lee YL
AD  - Department of Microbiology and Immunology, College of Medicine, School of Medical 
      Laboratory Science and Biotechnology, Taipei, Taiwan; Department of Pharmacology, 
      College of Medicine, School of Medical Laboratory Science and Biotechnology, 
      Taipei, Taiwan; College of Oral Medicine, Taipei Medical University, Taipei, 
      Taiwan; Department of Dentistry, National Yang-Ming University, Taipei, Taiwan; 
      Department of Family Medicine, Taipei Medical University, Wan Fang Hospital, 
      Taipei, Taiwan; Research Department, Human Protein Process Science, Lille, 
      France.
FAU - Lee, Lin-Wen
AU  - Lee LW
FAU - Su, Chen-Yao
AU  - Su CY
FAU - Hsiao, George
AU  - Hsiao G
FAU - Yang, Yi-Yuan
AU  - Yang YY
FAU - Leu, Sy-Jye
AU  - Leu SJ
FAU - Shieh, Ying-Hua
AU  - Shieh YH
FAU - Burnouf, Thierry
AU  - Burnouf T
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20130111
PL  - United States
TA  - Transfusion
JT  - Transfusion
JID - 0417360
RN  - 0 (Detergents)
RN  - 0 (Solvents)
SB  - IM
MH  - Animals
MH  - Asthma/immunology/metabolism/*therapy
MH  - Blood Platelets/drug effects/*virology
MH  - Detergents/pharmacology
MH  - Disease Models, Animal
MH  - Enzyme-Linked Immunosorbent Assay
MH  - Humans
MH  - Mice
MH  - Platelet Transfusion
MH  - Solvents/pharmacology
MH  - T-Lymphocytes, Regulatory/*immunology
EDAT- 2013/01/12 06:00
MHDA- 2013/11/13 06:00
CRDT- 2013/01/12 06:00
PHST- 2012/10/03 00:00 [received]
PHST- 2012/11/07 00:00 [revised]
PHST- 2012/11/12 00:00 [accepted]
PHST- 2013/01/12 06:00 [entrez]
PHST- 2013/01/12 06:00 [pubmed]
PHST- 2013/11/13 06:00 [medline]
AID - 10.1111/trf.12068 [doi]
PST - ppublish
SO  - Transfusion. 2013 Sep;53(9):1918-28. doi: 10.1111/trf.12068. Epub 2013 Jan 11.