PMID- 23305512 OWN - NLM STAT- MEDLINE DCOM- 20130909 LR - 20161125 IS - 2169-141X (Electronic) IS - 2169-1401 (Linking) VI - 41 IP - 2 DP - 2013 Apr TI - Characterization and cellular interaction of fluorescent-labeled PHEMA nanoparticles. PG - 78-84 LID - 10.3109/21691401.2012.742099 [doi] AB - The aim of this study is to label the cells with polymeric nanoparticles properly and efficiently. For this purpose, acridine orange (AO)-loaded poly(2-hydroxyethyl methacrylate) (PHEMA) nanoparticles were synthesized by miniemulsion polymerization. PHEMA nanoparticles were characterized by zeta sizer, zeta potential, atomic force microscopy (AFM), scanning electron microscopy (SEM), transmission electron microscopy (TEM), fluorescence microscopy, Fourier transform infrared spectrophotometer (FTIR), and elemental analysis. In addition, the toxicity of the nanoparticles were investigated by MTT [3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide] assay. FAU - Gokturk, Ilgim AU - Gokturk I AD - Department of Nanotechnology and Nanomedicine, Hacettepe University, Ankara-Turkey. FAU - Karakoc, Veyis AU - Karakoc V FAU - Onur, Mehmet Ali AU - Onur MA FAU - Denizli, Adil AU - Denizli A LA - eng PT - Journal Article DEP - 20130110 PL - England TA - Artif Cells Nanomed Biotechnol JT - Artificial cells, nanomedicine, and biotechnology JID - 101594777 RN - 0 (Drug Carriers) RN - 0 (Emulsions) RN - 0 (Fluorescent Dyes) RN - 0 (Tetrazolium Salts) RN - 0 (Thiazoles) RN - 25249-16-5 (Polyhydroxyethyl Methacrylate) RN - EUY85H477I (thiazolyl blue) RN - F30N4O6XVV (Acridine Orange) SB - IM MH - Acridine Orange MH - Animals MH - Cell Line MH - Cell Survival/drug effects MH - Drug Carriers/*chemical synthesis/pharmacology MH - Emulsions MH - Fibroblasts/cytology/*drug effects MH - Fluorescent Dyes MH - Mice MH - Microscopy, Atomic Force MH - Microscopy, Electron, Transmission MH - Nanoparticles/*chemistry/ultrastructure MH - Particle Size MH - Polyhydroxyethyl Methacrylate/*chemistry MH - Polymerization MH - Spectroscopy, Fourier Transform Infrared MH - Staining and Labeling MH - Tetrazolium Salts MH - Thiazoles EDAT- 2013/01/12 06:00 MHDA- 2013/09/10 06:00 CRDT- 2013/01/12 06:00 PHST- 2013/01/12 06:00 [entrez] PHST- 2013/01/12 06:00 [pubmed] PHST- 2013/09/10 06:00 [medline] AID - 10.3109/21691401.2012.742099 [doi] PST - ppublish SO - Artif Cells Nanomed Biotechnol. 2013 Apr;41(2):78-84. doi: 10.3109/21691401.2012.742099. Epub 2013 Jan 10.