PMID- 23306092
OWN - NLM
STAT- MEDLINE
DCOM- 20130726
LR  - 20130218
IS  - 1095-9947 (Electronic)
IS  - 1050-4648 (Linking)
VI  - 34
IP  - 3
DP  - 2013 Mar
TI  - Immune parameters correlating with reduced susceptibility to pancreas disease in 
      experimentally challenged Atlantic salmon (Salmo salar).
PG  - 789-98
LID - S1050-4648(12)00481-0 [pii]
LID - 10.1016/j.fsi.2012.12.014 [doi]
AB  - Two strains of Atlantic salmon (Salmon salar) with different susceptibility to 
      infectious salmon anaemia (ISA) were challenged with salmon pancreas disease 
      virus (SPDV), the etiological agent of salmon pancreas disease (PD), by 
      cohabitation. Serum and tissues were sampled at 0, 1, 3, 6 and 8 weeks 
      post-challenge. Experimental challenge with SAV did not cause mortality, but 
      virus loads and assessment of histopathology indicated that the fish more 
      resistant to ISAV (ISAHi) also was more resistant to PD. Eight weeks 
      post-challenge, the ISAHi strain had higher titres of SAV-neutralising antibodies 
      than the less resistant strain (ISALo). Transcript levels of four adaptive and 
      six innate immune parameters were analysed by real-time RT-PCR in heart, head 
      kidney (HK) and gills of both strains. Secretory IgM (sIgM) and CD8 levels 
      differed most between the two salmon strains. The ISAHi strain had significantly 
      higher levels of sIgM in HK at all samplings, and significantly higher CD8 levels 
      in gills at most samplings. In heart, both sIgM and CD8 levels increased 
      significantly during the challenge, but the increase appeared earlier for the 
      ISALo strain. By hierarchical clustering analysis of mRNA levels, a clear 
      segregation was observed between the two strains prior to the virus challenge. As 
      the viral infection developed, the clustering divide between fish strains 
      disappeared, first for innate and later for adaptive parameters. At eight weeks 
      post-challenge, the divide had however reformed for adaptive parameters. Possible 
      pair-wise correlation between transcript levels of immune parameters was 
      evaluated by a non-parametric statistical test. For innate parameters, the extent 
      of correlation peaked at 3 wpc in all tissues; this came rapidly for ISALo and 
      more gradual for ISAHi. The ISAHi strain tended to show higher correlation for 
      innate parameters in heart and gill than ISALo at early sampling times. For 
      adaptive immune parameters, little correlation was observed in general, except 
      for ISAHi in heart at 6 wpc. Overall, the observed differences in immune 
      parameters may provide important clues to the causes underlying the observed 
      difference in susceptibility to PD.
CI  - Copyright (c) 2013 Elsevier Ltd. All rights reserved.
FAU - Grove, Soren
AU  - Grove S
AD  - Section for Immunology, Norwegian Veterinary Institute, Oslo, Norway.
FAU - Austbo, Lars
AU  - Austbo L
FAU - Hodneland, Kjartan
AU  - Hodneland K
FAU - Frost, Petter
AU  - Frost P
FAU - Lovoll, Marie
AU  - Lovoll M
FAU - McLoughlin, Marian
AU  - McLoughlin M
FAU - Thim, Hanna L
AU  - Thim HL
FAU - Braaen, Stine
AU  - Braaen S
FAU - Konig, Melanie
AU  - Konig M
FAU - Syed, Mohasina
AU  - Syed M
FAU - Jorgensen, Jorunn B
AU  - Jorgensen JB
FAU - Rimstad, Espen
AU  - Rimstad E
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20130107
PL  - England
TA  - Fish Shellfish Immunol
JT  - Fish & shellfish immunology
JID - 9505220
SB  - IM
MH  - Adaptive Immunity
MH  - Alphavirus/*immunology/isolation & purification
MH  - Alphavirus Infections/genetics/immunology/*veterinary/virology
MH  - Animals
MH  - Disease Susceptibility/immunology/*veterinary/virology
MH  - Fish Diseases/genetics/*immunology/virology
MH  - Immunity, Innate
MH  - Isavirus/immunology/isolation & purification
MH  - Norway
MH  - Orthomyxoviridae Infections/genetics/immunology/veterinary/virology
MH  - Pancreatic Diseases/genetics/immunology/*veterinary/virology
MH  - Polymerase Chain Reaction/veterinary
MH  - *Salmo salar
EDAT- 2013/01/12 06:00
MHDA- 2013/07/28 06:00
CRDT- 2013/01/12 06:00
PHST- 2011/12/21 00:00 [received]
PHST- 2012/11/23 00:00 [revised]
PHST- 2012/12/10 00:00 [accepted]
PHST- 2013/01/12 06:00 [entrez]
PHST- 2013/01/12 06:00 [pubmed]
PHST- 2013/07/28 06:00 [medline]
AID - S1050-4648(12)00481-0 [pii]
AID - 10.1016/j.fsi.2012.12.014 [doi]
PST - ppublish
SO  - Fish Shellfish Immunol. 2013 Mar;34(3):789-98. doi: 10.1016/j.fsi.2012.12.014. 
      Epub 2013 Jan 7.