PMID- 23307868
OWN - NLM
STAT- MEDLINE
DCOM- 20130226
LR  - 20211203
IS  - 1549-5477 (Electronic)
IS  - 0890-9369 (Print)
IS  - 0890-9369 (Linking)
VI  - 27
IP  - 1
DP  - 2013 Jan 1
TI  - ATR-like kinase Mec1 facilitates both chromatin accessibility at DNA replication 
      forks and replication fork progression during replication stress.
PG  - 74-86
LID - 10.1101/gad.202978.112 [doi]
AB  - Faithful DNA replication is essential for normal cell division and 
      differentiation. In eukaryotic cells, DNA replication takes place on chromatin. 
      This poses the critical question as to how DNA replication can progress through 
      chromatin, which is inhibitory to all DNA-dependent processes. Here, we developed 
      a novel genome-wide method to measure chromatin accessibility to micrococcal 
      nuclease (MNase) that is normalized for nucleosome density, the NCAM (normalized 
      chromatin accessibility to MNase) assay. This method enabled us to discover that 
      chromatin accessibility increases specifically at and ahead of DNA replication 
      forks in normal S phase and during replication stress. We further found that 
      Mec1, a key regulatory ATR-like kinase in the S-phase checkpoint, is required for 
      both normal chromatin accessibility around replication forks and replication fork 
      rate during replication stress, revealing novel functions for the kinase in 
      replication stress response. These results suggest a possibility that Mec1 may 
      facilitate DNA replication fork progression during replication stress by 
      increasing chromatin accessibility around replication forks.
FAU - Rodriguez, Jairo
AU  - Rodriguez J
AD  - Fred Hutchinson Cancer Research Center, Division of Basic Sciences, Seattle, 
      Washington 98109, USA.
FAU - Tsukiyama, Toshio
AU  - Tsukiyama T
LA  - eng
GR  - R01 GM078259/GM/NIGMS NIH HHS/United States
GR  - R01 GM058465/GM/NIGMS NIH HHS/United States
GR  - ImNIH/Intramural NIH HHS/United States
GR  - GM078259/GM/NIGMS NIH HHS/United States
GR  - GM058465/GM/NIGMS NIH HHS/United States
PT  - Journal Article
PT  - Research Support, N.I.H., Intramural
PT  - Research Support, Non-U.S. Gov't
PL  - United States
TA  - Genes Dev
JT  - Genes & development
JID - 8711660
RN  - 0 (Chromatin)
RN  - 0 (Intracellular Signaling Peptides and Proteins)
RN  - 0 (Nucleosomes)
RN  - 0 (Saccharomyces cerevisiae Proteins)
RN  - EC 2.7.11.1 (MEC1 protein, S cerevisiae)
RN  - EC 2.7.11.1 (Protein Serine-Threonine Kinases)
RN  - EC 3.1.31.1 (Micrococcal Nuclease)
SB  - IM
MH  - Chromatin/chemistry/*metabolism
MH  - Chromosome Mapping
MH  - *DNA Replication
MH  - Genome/genetics
MH  - Intracellular Signaling Peptides and Proteins/genetics/*metabolism
MH  - Micrococcal Nuclease/metabolism
MH  - Mutation/genetics
MH  - Nucleosomes/metabolism
MH  - Protein Serine-Threonine Kinases/genetics/*metabolism
MH  - S Phase/genetics
MH  - Saccharomyces cerevisiae/*enzymology/*genetics
MH  - Saccharomyces cerevisiae Proteins/genetics/*metabolism
MH  - Stress, Physiological
PMC - PMC3553285
EDAT- 2013/01/12 06:00
MHDA- 2013/02/27 06:00
PMCR- 2013/07/01
CRDT- 2013/01/12 06:00
PHST- 2013/01/12 06:00 [entrez]
PHST- 2013/01/12 06:00 [pubmed]
PHST- 2013/02/27 06:00 [medline]
PHST- 2013/07/01 00:00 [pmc-release]
AID - 27/1/74 [pii]
AID - 10.1101/gad.202978.112 [doi]
PST - ppublish
SO  - Genes Dev. 2013 Jan 1;27(1):74-86. doi: 10.1101/gad.202978.112.