PMID- 23308358
OWN - NLM
STAT- MEDLINE
DCOM- 20131203
LR  - 20211021
IS  - 1520-5118 (Electronic)
IS  - 0021-8561 (Print)
IS  - 0021-8561 (Linking)
VI  - 61
IP  - 8
DP  - 2013 Feb 27
TI  - Effects of pregnane glycosides on food intake depend on stimulation of the 
      melanocortin pathway and BDNF in an animal model.
PG  - 1841-9
LID - 10.1021/jf3033649 [doi]
AB  - Pregnane glycosides appear to modulate food intake by possibly affecting the 
      hypothalamic feeding circuits; however, the mechanisms of the appetite-regulating 
      effect of pregnane glycosides remain obscure. Here, we show that pregnane 
      glycoside-enriched extracts from swamp milkweed Asclepias incarnata at 25-100 
      mg/kg daily attenuated food intake (up to 47.1 +/- 8.5% less than controls) and 
      body weight gain in rats (10% for males and 9% for females, respectively) by 
      activating melanocortin signaling and inhibiting gastric emptying. The major 
      milkweed pregnane glycoside, ikemagenin, exerted its appetite-regulating effect 
      by decreasing levels of agouti-related protein (0.6-fold) but not NPY satiety 
      peptides. Ikemagenin treatment also increased secretion of brain-derived 
      neurotropic factor (BDNF) downstream of melanocortin receptors in the 
      hypothalamus (1.4-fold) and in the C6 rat glioma cell culture in vitro (up to 
      6-fold). These results support the multimodal effects of pregnane glycosides on 
      feeding regulation, which depends on the activity of the melanocortin signaling 
      pathway and BDNF.
FAU - Komarnytsky, Slavko
AU  - Komarnytsky S
AD  - Plants for Human Health Institute, North Carolina State University, Kannapolis, 
      North Carolina 28081, United States. komarnytsky@ncsu.edu
FAU - Esposito, Debora
AU  - Esposito D
FAU - Rathinasabapathy, Thirumurugan
AU  - Rathinasabapathy T
FAU - Poulev, Alexander
AU  - Poulev A
FAU - Raskin, Ilya
AU  - Raskin I
LA  - eng
GR  - P50 AT002776/AT/NCCIH NIH HHS/United States
GR  - 2P50AT002776-06/AT/NCCIH NIH HHS/United States
PT  - Journal Article
PT  - Research Support, N.I.H., Extramural
DEP - 20130217
PL  - United States
TA  - J Agric Food Chem
JT  - Journal of agricultural and food chemistry
JID - 0374755
RN  - 0 (Brain-Derived Neurotrophic Factor)
RN  - 0 (Glycosides)
RN  - 0 (Melanocortins)
RN  - 0 (Plant Extracts)
RN  - 0 (Pregnanes)
RN  - 0 (pregnane glycoside)
SB  - IM
MH  - Animals
MH  - Appetite/drug effects
MH  - Asclepias/*chemistry
MH  - Brain-Derived Neurotrophic Factor/genetics/*metabolism
MH  - Cell Line, Tumor
MH  - Eating/*drug effects
MH  - Female
MH  - Glycosides/*administration & dosage
MH  - Humans
MH  - Male
MH  - Melanocortins/*metabolism
MH  - Models, Animal
MH  - Plant Extracts/*administration & dosage
MH  - Pregnanes/*administration & dosage
MH  - Rats
MH  - Rats, Wistar
PMC - PMC3805381
MID - NIHMS516906
COIS- The authors declare no competing financial interest.
EDAT- 2013/01/12 06:00
MHDA- 2013/12/16 06:00
PMCR- 2014/02/27
CRDT- 2013/01/12 06:00
PHST- 2013/01/12 06:00 [entrez]
PHST- 2013/01/12 06:00 [pubmed]
PHST- 2013/12/16 06:00 [medline]
PHST- 2014/02/27 00:00 [pmc-release]
AID - 10.1021/jf3033649 [doi]
PST - ppublish
SO  - J Agric Food Chem. 2013 Feb 27;61(8):1841-9. doi: 10.1021/jf3033649. Epub 2013 
      Feb 17.