PMID- 23312853
OWN - NLM
STAT- MEDLINE
DCOM- 20130722
LR  - 20220310
IS  - 1878-5905 (Electronic)
IS  - 0142-9612 (Print)
IS  - 0142-9612 (Linking)
VI  - 34
IP  - 9
DP  - 2013 Mar
TI  - A genomics approach in determining nanotopographical effects on MSC phenotype.
PG  - 2177-84
LID - S0142-9612(12)01399-3 [pii]
LID - 10.1016/j.biomaterials.2012.12.019 [doi]
AB  - Topography and its effects on cell adhesion, morphology, growth and 
      differentiation are well documented. Thus, current advances with the use of 
      nanotopographies offer promising results in the field of regenerative medicine. 
      Studies have also shown nanotopographies to have strong effects on stem cell 
      self-renewal and differentiation. What is less clear however is what 
      mechanotransductive mechanisms are employed by the cells to facilitate such 
      changes. In fastidious cell types, it has been suggested that direct 
      mechanotransduction producing morphological changes in the nucleus, 
      nucleoskeleton and chromosomes themselves may be central to cell responses to 
      topography. In this report we move these studies into human skeletal or 
      mesenchymal stem cells and propose that direct (mechanical) signalling is 
      important in the early stages of tuning stem cell fate to nanotopography. Using 
      fluorescence in situ hybridization (FISH) and Affymetrix arrays we have evidence 
      that nanotopography stimulates changes in nuclear organisation that can be linked 
      to spatially regulated genes expression with a particular focus on phenotypical 
      genes. For example, chromosome 1 was seen to display the largest numbers of gene 
      deregulations and also a concomitant change in nuclear positioning in response to 
      nanotopography. Plotting of deregulated genes in reference to band positioning 
      showed that topographically related changes tend to happen towards the telomeric 
      ends of the chromosomes, where bone related genes are generally clustered. Such 
      an approach offers a better understanding of cell-surface interaction and, 
      critically, provides new insights of how to control stem cell differentiation 
      with future applications in areas including regenerative medicine.
CI  - Copyright (c) 2012 Elsevier Ltd. All rights reserved.
FAU - Tsimbouri, Penelope M
AU  - Tsimbouri PM
AD  - Centre for Cell Engineering, Institute of Molecular, Cell and Systems Biology, 
      College of Medical, Veterinary and Life Sciences, University of Glasgow, Glasgow 
      G12 8QQ, Scotland, UK. penelope.tsimbouri@glasgow.ac.uk
FAU - Murawski, Kate
AU  - Murawski K
FAU - Hamilton, Graham
AU  - Hamilton G
FAU - Herzyk, Pawel
AU  - Herzyk P
FAU - Oreffo, Richard O C
AU  - Oreffo RO
FAU - Gadegaard, Nikolaj
AU  - Gadegaard N
FAU - Dalby, Matthew J
AU  - Dalby MJ
LA  - eng
GR  - BB/G006970/1/BB_/Biotechnology and Biological Sciences Research Council/United 
      Kingdom
GR  - BB/G008868/1/BB_/Biotechnology and Biological Sciences Research Council/United 
      Kingdom
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20130109
PL  - Netherlands
TA  - Biomaterials
JT  - Biomaterials
JID - 8100316
SB  - IM
MH  - Blotting, Western
MH  - Cell Adhesion
MH  - Cell Differentiation
MH  - Cells, Cultured
MH  - Gene Expression Regulation
MH  - Genomics/*methods
MH  - Humans
MH  - In Situ Hybridization, Fluorescence
MH  - Mechanotransduction, Cellular
MH  - Mesenchymal Stem Cells/*cytology
MH  - Nanotechnology/*methods
MH  - *Phenotype
MH  - Real-Time Polymerase Chain Reaction
MH  - Regenerative Medicine/methods
PMC - PMC3573234
EDAT- 2013/01/15 06:00
MHDA- 2013/07/23 06:00
PMCR- 2013/03/01
CRDT- 2013/01/15 06:00
PHST- 2012/09/10 00:00 [received]
PHST- 2012/12/15 00:00 [accepted]
PHST- 2013/01/15 06:00 [entrez]
PHST- 2013/01/15 06:00 [pubmed]
PHST- 2013/07/23 06:00 [medline]
PHST- 2013/03/01 00:00 [pmc-release]
AID - S0142-9612(12)01399-3 [pii]
AID - 10.1016/j.biomaterials.2012.12.019 [doi]
PST - ppublish
SO  - Biomaterials. 2013 Mar;34(9):2177-84. doi: 10.1016/j.biomaterials.2012.12.019. 
      Epub 2013 Jan 9.