PMID- 2331307
OWN - NLM
STAT- MEDLINE
DCOM- 19900606
LR  - 20191029
IS  - 0926-5287 (Print)
IS  - 0926-5287 (Linking)
VI  - 30
IP  - 1
DP  - 1990
TI  - Effects of freezing-thawing on the spermatozoon nucleus: a comparative chromatin 
      cytophotometric study in the porcine and human species.
PG  - 59-64
AB  - Freezing-thawing effects on the nuclei of porcine and human spermatozoa were 
      studied by determining native DNA percentage from fluorescence after acridine 
      orange (AO) staining and by analyzing chromatin structure by a quantitative 
      microspectrophotometric study of Feulgen-DNA complexes before and after freezing. 
      The study of boar spermatozoa revealed no alteration in native DNA percentage 
      after freezing. However, native DNA percentage decreased significantly in human 
      spermatozoa. Feulgen-DNA content and sperm nuclear surface area decreased in both 
      species after freezing. These results prompted us to hypothesize an 
      overcondensation of sperm chromatin after freezing-thawing. This overcondensation 
      may be related to the lower conception rates obtained with human and porcine 
      semen after cryostorage via defective decondensation.
FAU - Hamamah, S
AU  - Hamamah S
AD  - Departement de Gynecologie-Obstetrique, Hopital Bretonneau, Tours, France.
FAU - Royere, D
AU  - Royere D
FAU - Nicolle, J C
AU  - Nicolle JC
FAU - Paquignon, M
AU  - Paquignon M
FAU - Lansac, J
AU  - Lansac J
LA  - eng
PT  - Comparative Study
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - France
TA  - Reprod Nutr Dev
JT  - Reproduction, nutrition, development
JID - 8913069
RN  - 0 (Chromatin)
RN  - 9007-49-2 (DNA)
SB  - IM
MH  - Animals
MH  - Cattle
MH  - Cell Nucleus/ultrastructure
MH  - Chromatin/*ultrastructure
MH  - *Cryopreservation
MH  - DNA/analysis
MH  - Humans
MH  - Male
MH  - Nucleic Acid Denaturation
MH  - *Semen Preservation
MH  - Species Specificity
MH  - Spermatozoa/analysis/*ultrastructure
MH  - Swine/*physiology
EDAT- 1990/01/01 00:00
MHDA- 1990/01/01 00:01
CRDT- 1990/01/01 00:00
PHST- 1990/01/01 00:00 [pubmed]
PHST- 1990/01/01 00:01 [medline]
PHST- 1990/01/01 00:00 [entrez]
AID - 10.1051/rnd:19900105 [doi]
PST - ppublish
SO  - Reprod Nutr Dev. 1990;30(1):59-64. doi: 10.1051/rnd:19900105.